ArticleBMC genomics2013
Genome analyses of the wheat yellow (stripe) rust pathogen Puccinia striiformis f. sp. tritici reveal polymorphic and haustorial expressed secreted proteins as candidate effectors.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 228 citations in OpenAlex.
- RNA-Seq Profiling Identifies Temperature-Responsive Genes in Germinating Urediniospores ofJournal of fungi (Basel, Switzerland) · 2026Article
- Two divergent haploid nuclei shaped the landscape of population diversity in wheat stripe rust Puccinia striiformis f. sp. tritici.Communications biology · 2026Article
- Wheat's war against stripe rust: Integrating host immunity, genomics and breeding for durable resistance.The plant genome · 2026Review
- Virulence gains in the Puccinia striiformis f. sp. tritici PstS10 lineage correlate with expression polymorphism in a candidate Avr effector.Communications biology · 2026Article
- The proteome study of germinated Puccinia triticina urediniospores reveals a novel effector protein required for virulence.Scientific reports · 2026Article
- Avirulence genes identified through linkage mapping and region-specific association studies in the wheat leaf rust pathogen Puccinia triticina.BMC genomics · 2026Article
- Integrated Transcriptomic and Metabolomic Analysis Reveals Molecular Signatures Associated with Natural Degeneration ofCurrent issues in molecular biology · 2026Article
- The putative effector BgtE-20069a, secreted from Blumeria graminis f. sp. tritici, suppresses plant immunity to facilitate wheat infection.BMC microbiology · 2025Article
- Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.Molecular plant pathology · 2025Review
- Differentiation of European yellow rust subraces within the 'Warrior(-)' genetic group.PloS one · 2025Article
- Quantitative phosphoproteomics reveals molecular pathway network in wheat resistance to stripe rust.Stress biology · 2024Article
- Molecular Mechanisms of the Stripe Rust Interaction with Resistant and Susceptible Wheat Genotypes.International journal of molecular sciences · 2024Article
- A bird's-eye view: exploration of the flavin-containing monooxygenase superfamily in common wheat.Frontiers in plant science · 2024Article
- Uncovering the history of recombination and population structure in western Canadian stripe rust populations through mating type alleles.BMC biology · 2023Article
- Clonal reproduction of Moniliophthora roreri and the emergence of unique lineages with distinct genomes during range expansion.G3 (Bethesda, Md.) · 2023Article
- High-throughput RNA sequencing reveals differences between the transcriptomes of the five spore forms of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen.Stress biology · 2023Article
- Transcriptome analysis ofFrontiers in microbiology · 2023Article
- Functional Analyses of a Small Secreted Cysteine-Rich Protein ThSCSP_14 inInternational journal of molecular sciences · 2022Article
- ThSCSP_12: Novel Effector inInternational journal of molecular sciences · 2022Article
- Current Status and Future Perspectives of Genomics Research in the Rust Fungi.International journal of molecular sciences · 2022Review
53 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundWheat yellow (stripe) rust caused by Puccinia striiformis f. sp. tritici (PST) is one of the most devastating diseases of wheat worldwide. To design effective breeding strategies that maximize the potential for durable disease resistance it is important to understand the molecular basis of PST pathogenicity. In particular, the characterisation of the structure, function and evolutionary dynamics of secreted effector proteins that are detected by host immune receptors can help guide and prioritize breeding efforts. However, to date, our knowledge of the effector repertoire of cereal rust pathogens is limited.
resultsWe re-sequenced genomes of four PST isolates from the US and UK to identify effector candidates and relate them to their distinct virulence profiles. First, we assessed SNP frequencies between all isolates, with heterokaryotic SNPs being over tenfold more frequent (5.29 ± 2.23 SNPs/kb) than homokaryotic SNPs (0.41 ± 0.28 SNPs/kb). Next, we implemented a bioinformatics pipeline to integrate genomics, transcriptomics, and effector-focused annotations to identify and classify effector candidates in PST. RNAseq analysis highlighted transcripts encoding secreted proteins that were significantly enriched in haustoria compared to infected tissue. The expression of 22 candidate effector genes was characterised using qRT-PCR, revealing distinct temporal expression patterns during infection in wheat. Lastly, we identified proteins that displayed non-synonymous substitutions specifically between the two UK isolates PST-87/7 and PST-08/21, which differ in virulence to two wheat varieties. By focusing on polymorphic variants enriched in haustoria, we identified five polymorphic effector candidates between PST-87/7 and PST-08/21 among 2,999 secreted proteins. These allelic variants are now a priority for functional validation as virulence/avirulence effectors in the corresponding wheat varieties.
conclusionsIntegration of genomics, transcriptomics, and effector-directed annotation of PST isolates has enabled us to move beyond the single isolate-directed catalogues of effector proteins and develop a framework for mining effector proteins in closely related isolates and relate these back to their defined virulence profiles. This should ultimately lead to more comprehensive understanding of the PST pathogenesis system, an important first step towards developing more effective surveillance and management strategies for one of the most devastating pathogens of wheat.
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