ArticleJournal of biological engineering2013
Wide-dynamic-range promoters engineered for cyanobacteria.
Article in Journal of biological engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Context-aware synthetic promoter design using neural networks enables rewiring of eukaryotic transcriptional networks.NPJ systems biology and applications · 2026Article
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- A Cyanobacterial Screening Platform for Rubisco Mutant Variants.ACS synthetic biology · 2025Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Fine-Tuning Gene Expression in Bacteria by Synthetic Promoters.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2023Article
- The organization of the phycobilisome-photosystem I supercomplex depends on the ratio between two different phycobilisome linker proteins.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Construction of pSM201v: A broad host range replicative vector based on shortening of RSF1010.Heliyon · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- Bubble Relaxation Dynamics in Homopolymer DNA Sequences.Molecules (Basel, Switzerland) · 2023Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- Approaches in the photosynthetic production of sustainable fuels by cyanobacteria using tools of synthetic biology.World journal of microbiology & biotechnology · 2021Review
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
- A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria.Marine biotechnology (New York, N.Y.) · 2021Article
- Article
- The Effect of Promoter and RBS Combination on the Growth and Glycogen Productivity of Sodium-Dependent Bicarbonate Transporter (SbtA) OverexpressingFrontiers in microbiology · 2021Article
- Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Recent advances in synthetic biology of cyanobacteria for improved chemicals production.Bioengineered · 2020Review
- Improving heterologous protein expression inMetabolic engineering communications · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCyanobacteria, prokaryotic cells with oxygenic photosynthesis, are excellent bioengineering targets to convert solar energy into solar fuels. Tremendous genetic engineering approaches and tools have been and still are being developed for prokaryotes. However, the progress for cyanobacteria is far behind with a specific lack of non-native inducible promoters.
resultsWe report the development of engineered TetR-regulated promoters with a wide dynamic range of transcriptional regulation. An optimal 239 (±16) fold induction in darkness (white-light-activated heterotrophic growth, 24 h) and an optimal 290 (±93) fold induction in red light (photoautotrophic growth, 48 h) were observed with the L03 promoter in cells of the unicellular cyanobacterium Synechocystis sp. strain ATCC27184 (i.e. glucose-tolerant Synechocystis sp. strain PCC 6803). By altering only few bases of the promoter in the narrow region between the -10 element and transcription start site significant changes in the promoter strengths, and consequently in the range of regulations, were observed.
conclusionsThe non-native inducible promoters developed in the present study are ready to be used to further explore the notion of custom designed cyanobacterial cells in the complementary frameworks of metabolic engineering and synthetic biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.