ArticleJournal of natural products2013
Genetic insights into pyralomicin biosynthesis in Nonomuraea spiralis IMC A-0156.
Article in Journal of natural products, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- iPRESTO: Automated discovery of biosynthetic sub-clusters linked to specific natural product substructures.PLoS computational biology · 2023Article
- Biochemical Characterization of GacI, a Bifunctional Glycosyltransferase-Phosphatase Enzyme Involved in Acarbose Biosynthesis inBiochemistry · 2022Article
- Complete biosynthetic pathway to the antidiabetic drug acarbose.Nature communications · 2022Article
- Discovery and Biosynthesis of Tetrachlorizine Reveals Enzymatic Benzylic Dehydrogenation via anJournal of the American Chemical Society · 2021Article
- Natural Occurrence of Hybrid Polyketides from Two Distinct Biosynthetic Pathways inACS chemical biology · 2021Article
- Type II non-ribosomal peptide synthetase proteins: structure, mechanism, and protein-protein interactions.Natural product reports · 2020Review
- Mzabimycins A and B, novel intracellular angucycline antibiotics produced bySaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2019Article
- The sedoheptulose 7-phosphate cyclases and their emerging roles in biology and ecology.Natural product reports · 2017Review
- Evolution and Distribution of CACS chemical biology · 2017Article
- A comprehensive review of glycosylated bacterial natural products.Chemical Society reviews · 2015Review
- Distinct Substrate Specificity and Catalytic Activity of the Pseudoglycosyltransferase VldE.Chemistry & biology · 2015Article
- Characterization of antibiotic producing rare actinomycete Nonomuraea sp. JAJ18 derived from an Indian coastal solar saltern.TheScientificWorldJournal · 2014Article
- Non-streptomycete actinomycetes nourish the current microbial antibiotic drug discovery.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
The biosynthetic gene cluster for the pyralomicin antibiotics has been cloned and sequenced from Nonomuraea spiralis IMC A-0156. The 41 kb gene cluster contains 27 ORFs predicted to encode all of the functions for pyralomicin biosynthesis. This includes nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) required for the formation of the benzopyranopyrrole core unit, as well as a suite of tailoring enzymes (e.g., four halogenases, an O-methyltransferase, and an N-glycosyltransferase) necessary for further modifications of the core structure. The N-glycosyltransferase is predicted to transfer either glucose or a pseudosugar (cyclitol) to the aglycone. A gene cassette encoding C7-cyclitol biosynthetic enzymes was identified upstream of the benzopyranopyrrole-specific ORFs. Targeted disruption of the gene encoding the N-glycosyltransferase, prlH, abolished pyralomicin production, and recombinant expression of PrlA confirms the activity of this enzyme as a sugar phosphate cyclase involved in the formation of the C7-cyclitol moiety.
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