ArticleBioMed research international2013
In vitro construction of scaffold-free bilayered tissue-engineered skin containing capillary networks.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Integrating melt electrowriting (MEW) PCL scaffolds with fibroblast-laden hydrogel toward vascularized skin tissue engineering.Materials today. Bio · 2025Article
- From 1D microbiological assays to 3D advanced skin models: enhancing preclinical strategies to unravel the impact of bioactive textiles on the human skin microbiome.Frontiers in cellular and infection microbiology · 2025Review
- Human organoids in basic research and clinical applications.Signal transduction and targeted therapy · 2022Review
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Dental Pulp Cell Sheets Enhance Facial Nerve Regeneration via Local Neurotrophic Factor Delivery.Tissue engineering. Part A · 2021Article
- Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates.The Journal of international medical research · 2020Observational
- A biofabricated vascularized skin model of atopic dermatitis for preclinical studies.Biofabrication · 2020Article
- Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering.International journal of nanomedicine · 2019Review
- Functional Skin Grafts: Where Biomaterials Meet Stem Cells.Stem cells international · 2019Review
- Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development.Scientific reports · 2018Article
- Scaffold-free tissue engineering of functional corneal stromal tissue.Journal of tissue engineering and regenerative medicine · 2018Article
- Autologous fibroblasts, peripheral blood mononuclear cells, and fibrin glue accelerate healing of refractory cutaneous ulcers in diabetic mice.American journal of translational research · 2018Article
- Ulcer healing effect of autologous mixed sheets consisting of fibroblasts and peripheral blood mononuclear cells in rabbit ischemic hind limb.American journal of translational research · 2017Article
- Methodologies in creating skin substitutes.Cellular and molecular life sciences : CMLS · 2016Review
- Behaviour of endothelial cells in a tridimensional in vitro environment.BioMed research international · 2015Article
- The effects of constant flow bioreactor cultivation and keratinocyte seeding densities on prevascularized organotypic skin grafts based on a fibrin scaffold.Tissue engineering. Part A · 2015Article
- Tissue-engineered skin substitutes: an overview.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2013Review
- Current progress of skin tissue engineering: Seed cells, bioscaffolds, and construction strategies.Burns & trauma · 2013Article
- Peptide nanogels as a scaffold for fabricating dermal grafts and 3D vascularized skin models.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many types of skin substitutes have been constructed using exogenous materials. Angiogenesis is an important factor for tissue-engineered skin constructs. In this study, we constructed a scaffold-free bilayered tissue-engineered skin containing a capillary network. First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. A fibrous sheet was formed by the interactions between the fibroblasts and the endothelial cells, and capillary-like structures were observed after 20 days of coculture. Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. HE staining showed that tissue-engineered skin exhibited a stratified epidermis after 7 days. Immunostaining showed that the epithelium promoted the formation of capillary-like structures. Transmission electron microscopy (TEM) analysis showed that the capillary-like structures were typical microblood vessels. ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.