ArticleMycotoxin research2009
A strain of Fusarium kyushuense is able to produce aflatoxin B1 and G 1.
Article in Mycotoxin research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Article
- The genome of newly classified Ochroconis mirabilis: Insights into fungal adaptation to different living conditions.BMC genomics · 2016Article
- Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A strain of F. kyushuense is able to produce aflatoxin B1 and G1 under certain growth conditions. This was ultimately demonstrated by LC-MS analysis. By using a microarray covering oligonucleotides specific for the aflatoxin pathway genes of A. flavus, the expression of homologues of nearly the complete aflatoxin biosynthesis gene cluster was detected in F. kyushuense under producing conditions, e.g., after growth in liquid YES medium. In contrast, after growth of F. kyushuense on solid YES medium, only a very low transcription level of one of the homologues (nor-1) could be observed by microarray and Real Time PCR demonstrating differential gene expression. The cross-hybridization of the F. kyushuense mRNA to the A. flavus aflatoxin biosynthesis gene oligonucleotides of the array indicates homology between the sequences. PCR analyses with primers deduced from the nor-1 gene sequence of the aflatoxin biosynthesis gene cluster from A. flavus revealed the presence of a homologue of this gene in F. kyushuense. Sequencing of PCR fragments of the nor-1 homologue revealed a sequence similarity of >90% to the nor-1 gene of A. flavus.
Identifiers
23605093What OpenQuestion holds
Registered trials
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