ArticleNucleic acids research2013
The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Epitranscriptomic profiling of cytosine N4 acetylation (acMolecular horticulture · 2026Article
- Proximal proteomics reveals a landscape of human nuclear condensates.Nature cell biology · 2025Article
- Review
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
- Review
- An Arabidopsis Retention and Splicing complex regulates root and embryo development through pre-mRNA splicing.Plant physiology · 2022Article
- A Census and Categorization Method of Epitranscriptomic Marks.International journal of molecular sciences · 2020Review
- The Processing, Gene Regulation, Biological Functions, and Clinical Relevance of N4-Acetylcytidine on RNA: A Systematic Review.Molecular therapy. Nucleic acids · 2020Review
- Phytochemical and pharmacological screening ofFood science and biotechnology · 2018Article
- RES complex is associated with intron definition and required for zebrafish early embryogenesis.PLoS genetics · 2018Article
- Identification of factors that promote biogenesis of tRNARNA biology · 2018Article
- The pre-mRNA retention and splicing complex controls expression of the Mediator subunit Med20.RNA biology · 2017Article
- Resources for the Comprehensive Discovery of Functional RNA Elements.Molecular cell · 2016Article
- Article
- Retention and splicing complex (RES) - the importance of cooperativity.RNA biology · 2016Article
- Structures of intermediates during RES complex assembly.Scientific reports · 2015Article
- Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.PLoS genetics · 2015Article
- A novel protein-protein interaction in the RES (REtention and Splicing) complex.The Journal of biological chemistry · 2014Article
- Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.Nature structural & molecular biology · 2014Article
- Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae.PLoS genetics · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conserved pre-mRNA retention and splicing (RES) complex, which in yeast consists of Bud13p, Snu17p and Pml1p, is thought to promote nuclear retention of unspliced pre-mRNAs and enhance splicing of a subset of transcripts. Here, we find that the absence of Bud13p or Snu17p causes greatly reduced levels of the modified nucleoside N(4)-acetylcytidine (ac(4)C) in tRNA and that a lack of Pml1p reduces ac(4)C levels at elevated temperatures. The ac(4)C nucleoside is normally found at position 12 in the tRNA species specific for serine and leucine. We show that the tRNA modification defect in RES-deficient cells is attributable to inefficient splicing of TAN1 pre-mRNA and the effects of reduced Tan1p levels on formation of ac(4)C. Analyses of cis-acting elements in TAN1 pre-mRNA showed that the intron sequence between the 5' splice site and branchpoint is necessary and sufficient to mediate RES dependency. We also show that in RES-deficient cells, the TAN1 pre-mRNA is targeted for degradation by the cytoplasmic nonsense-mediated mRNA decay pathway, indicating that poor nuclear retention may contribute to the tRNA modification defect. Our results demonstrate that TAN1 pre-mRNA processing has an unprecedented requirement for RES factors and that the complex controls the formation of ac(4)C in tRNA.
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