ArticleNucleic acids research2013
Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- Adaptive genetics reveals constraints on protein structure/function by evolving E. coli under constant nutrient limitation.BMC biology · 2025Article
- Models of Hfq interactions with small non-coding RNA in Gram-negative and Gram-positive bacteria.Frontiers in cellular and infection microbiology · 2023Review
- Genetically encoded chemical crosslinking of RNA in vivo.Nature chemistry · 2023Article
- Post-Transcriptional Control in the Regulation of Polyhydroxyalkanoates Synthesis.Life (Basel, Switzerland) · 2021Review
- Differential Chromosome- and Plasmid-Borne Resistance ofInternational journal of molecular sciences · 2021Article
- PresRAT: a server for identification of bacterial small-RNA sequences and their targets with probable binding region.RNA biology · 2021Article
- Engineering of the Small Noncoding RNA (sRNA) DsrA Together with the sRNA Chaperone Hfq Enhances the Acid Tolerance of Escherichia coli.Applied and environmental microbiology · 2021Article
- Crystal structure of an Escherichia coli Hfq Core (residues 2-69)-DNA complex reveals multifunctional nucleic acid binding sites.Nucleic acids research · 2020Article
- Article
- A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo.Nucleic acids research · 2018Article
- Intermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq.Scientific reports · 2017Article
- Balancing gene expression without library construction via a reusable sRNA pool.Nucleic acids research · 2017Article
- Functional analyses of putative PalS (Palindromic Self-recognition) motifs in bacterial Hfq.Biochemical and biophysical research communications · 2017Article
- Crystal structure and RNA-binding properties of an Hfq homolog from the deep-branching Aquificae: conservation of the lateral RNA-binding mode.Acta crystallographica. Section D, Structural biology · 2017Article
- Atomistic modelling of scattering data in the Collaborative Computational Project for Small Angle Scattering (CCP-SAS).Journal of applied crystallography · 2016Article
- Hfq: the flexible RNA matchmaker.Current opinion in microbiology · 2016Review
- Alternative Hfq-sRNA interaction modes dictate alternative mRNA recognition.The EMBO journal · 2015Article
- Structural insights into the recognition of the internal A-rich linker from OxyS sRNA by Escherichia coli Hfq.Nucleic acids research · 2015Article
- Article
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5 authors.
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Abstract
The rpoS mRNA, which encodes the master regulator σ(S) of general stress response, requires Hfq-facilitated base pairing with DsrA small RNA for efficient translation at low temperatures. It has recently been proposed that one mechanism underlying Hfq action is to bridge a transient ternary complex by simultaneously binding to rpoS and DsrA. However, no structural evidence of Hfq simultaneously bound to different RNAs has been reported. We detected simultaneous binding of Hfq to rpoS and DsrA fragments. Crystal structures of AU6A•Hfq•A7 and Hfq•A7 complexes were resolved using 1.8- and 1.9-Å resolution, respectively. Ternary complex has been further verified in solution by NMR. In vivo, activation of rpoS translation requires intact Hfq, which is capable of bridging rpoS and DsrA simultaneously into ternary complex. This ternary complex possibly corresponds to a meta-stable transition state in Hfq-facilitated small RNA-mRNA annealing process.
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