Trial reportAnnals of biomedical engineering2013
Isolation of functional human endothelial cells from small volumes of umbilical cord blood.
Trial report in Annals of biomedical engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Engineered myovascular tissues for studies of endothelial/satellite cell interactions.Acta biomaterialia · 2024Article
- Development of In Vitro Endothelialised Stents - Review.Stem cell reviews and reports · 2022Review
- Modeling early stage atherosclerosis in a primary human vascular microphysiological system.Nature communications · 2020Article
- Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells.Journal of clinical medicine · 2019Article
- Pro-apoptotic effect of TRAIL-transfected endothelial progenitor cells on glioma cells.Oncology letters · 2018Article
- Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord.Experimental and therapeutic medicine · 2018Article
- Accelerated oral wound healing using a pre-vascularized mucosal cell sheet.Scientific reports · 2017Article
- Point-of-care seeding of nitinol stents with blood-derived endothelial cells.Journal of biomedical materials research. Part B, Applied biomaterials · 2016Article
- Efficient One-Step Production of Microencapsulated Hepatocyte Spheroids with Enhanced Functions.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Increased yield of endothelial cells from peripheral blood for cell therapies and tissue engineering.Regenerative medicine · 2015Article
- Article
- Biological and engineering design considerations for vascular tissue engineered blood vessels (TEBVs).Current opinion in chemical engineering · 2014Article
- Late-outgrowth endothelial progenitors from patients with coronary artery disease: endothelialization of confluent stromal cell layers.Acta biomaterialia · 2014Article
- Point-of-Care Rapid-Seeding Ventricular Assist Device with Blood-Derived Endothelial Cells to Create a Living Antithrombotic Coating.ASAIO journal (American Society for Artificial Internal Organs : 1992)Article
- Regeneration in Experimental Alveolar Bone Defect Using Human Umbilical Cord Mesenchymal Stem Cells.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Endothelial cells (ECs) isolated from endothelial progenitor cells in blood have great potential as a therapeutic tool to promote vasculogenesis and angiogenesis and treat cardiovascular diseases. However, current methods to isolate ECs are limited by a low yield with few colonies appearing during isolation. In order to utilize blood-derived ECs for therapeutic applications, a simple method is needed that can produce a high yield of ECs from small volumes of blood without the addition of animal-derived products. For the first time, we show that human ECs can be isolated without the prior separation of blood components through the technique of diluted whole blood incubation (DWBI) utilizing commercially available human serum. We isolated ECs from small volumes of blood (~10 mL) via DWBI and characterized them with flow cytometry, immunohistochemistry, and uptake of DiI-labeled acetylated low density lipoprotein (DiI-Ac-LDL). These ECs are functional as demonstrated by their ability to form tubular networks in Matrigel, adhere and align with flow under physiological fluid shear stress, and produce increased nitric oxide under fluid flow. An average of 7.0 ± 2.5 EC colonies that passed all functional tests described above were obtained per 10 mL of blood as compared to only 0.3 ± 0.1 colonies with the traditional method based on density centrifugation. The time until first colony appearance was 8.3 ± 1.2 days for ECs isolated with the DWBI method and 12 ± 1.4 days for ECs isolated with the traditional isolation method. A simplified method, such as DWBI, in combination with advances in isolation yield could enable the use of blood-derived ECs in clinical practice.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.