ArticleIntegrative and comparative biology2013
Anaerobic metabolism at thermal extremes: a metabolomic test of the oxygen limitation hypothesis in an aquatic insect.
Article in Integrative and comparative biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 100 citations in OpenAlex.
- These Boots Are Made for Walking: Sex-Specific Physiological and Metabolomic Strategies Reflect Male-Skewed Vulnerability to Ocean Warming in a Keystone Amphipod.Global change biology · 2026Article
- Does heat tolerance vary with rates of oxygen production in photosymbiotic cnidarians?The Journal of experimental biology · 2026Article
- Thermal tolerance of giant salmonfly nymphs (Conservation physiology · 2024Article
- The Multifaceted Effects of Short-Term Acute Hypoxia Stress: Insights into the Tolerance Mechanism ofInsects · 2023Article
- Metabolic plasticity improves lobster's resilience to ocean warming but not to climate-driven novel species interactions.Scientific reports · 2022Article
- Temperature and Diet Acclimation Modify the Acute Thermal Performance of the Largest Extant Amphibian.Animals : an open access journal from MDPI · 2022Article
- Assessing the PThe Science of the total environment · 2022Article
- Flexible Thermal Sensitivity of Mitochondrial Oxygen Consumption and Substrate Oxidation in Flying Insect Species.Frontiers in physiology · 2022Article
- Diel oxygen fluctuation drives the thermal response and metabolic performance of coastal marine ectotherms.Proceedings. Biological sciences · 2021Article
- Flow increases tolerance of heat and hypoxia of an aquatic insect.Biology letters · 2021Article
- Lower bioenergetic costs but similar immune responsiveness under a heat wave in urban compared to rural damselflies.Evolutionary applications · 2021Article
- Transcriptomic and life history responses of the mayfly Neocloeon triangulifer to chronic diel thermal challenge.Scientific reports · 2020Article
- It's all about the fluxes: Temperature influences ion transport and toxicity in aquatic insects.Aquatic toxicology (Amsterdam, Netherlands) · 2020Article
- Metabolic response ofPeerJ · 2020Article
- Scaling of thermal tolerance with body mass and genome size in ectotherms: a comparison between water- and air-breathers.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Hypoxia causes woodlice (Porcellio scaber) to select lower temperatures and impairs their thermal performance and heat tolerance.PloS one · 2019Article
- Elevated COMetabolomics : Official journal of the Metabolomic Society · 2018Article
- Transient window of resilience during early development minimizes teratogenic effects of heat in zebrafish embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- Thermal limits in native and alien freshwater peracarid Crustacea: The role of habitat use and oxygen limitation.Functional ecology · 2018Article
- A positive genetic correlation between hypoxia tolerance and heat tolerance supports a controversial theory of heat stress.Biology letters · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermal limits in ectotherms may arise through a mismatch between supply and demand of oxygen. At higher temperatures, the ability of their cardiac and ventilatory activities to supply oxygen becomes insufficient to meet their elevated oxygen demand. Consequently, higher levels of oxygen in the environment are predicted to enhance tolerance of heat, whereas reductions in oxygen are expected to reduce thermal limits. Here, we extend previous research on thermal limits and oxygen limitation in aquatic insect larvae and directly test the hypothesis of increased anaerobic metabolism and lower energy status at thermal extremes. We quantified metabolite profiles in stonefly nymphs under varying temperatures and oxygen levels. Under normoxia, the concept of oxygen limitation applies to the insects studied. Shifts in the metabolome of heat-stressed stonefly nymphs clearly indicate the onset of anaerobic metabolism (e.g., accumulation of lactate, acetate, and alanine), a perturbation of the tricarboxylic acid cycle (e.g., accumulation of succinate and malate), and a decrease in energy status (e.g., ATP), with corresponding decreases in their ability to survive heat stress. These shifts were more pronounced under hypoxic conditions, and negated by hyperoxia, which also improved heat tolerance. Perturbations of metabolic pathways in response to either heat stress or hypoxia were found to be somewhat similar but not identical. Under hypoxia, energy status was greatly compromised at thermal extremes, but energy shortage and anaerobic metabolism could not be conclusively identified as the sole cause underlying thermal limits under hyperoxia. Metabolomics proved useful for suggesting a range of possible mechanisms to explore in future investigations, such as the involvement of leaking membranes or free radicals. In doing so, metabolomics provided a more complete picture of changes in metabolism under hypoxia and heat stress.
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