ArticleCellular and molecular life sciences : CMLS2013
Two types of muscarinic acetylcholine receptors in Drosophila and other arthropods.
Article in Cellular and molecular life sciences : CMLS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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40 citing papers in PubMed, 76 citations in OpenAlex.
- Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinus.Nature communications · 2026Article
- Nonlinear high-activity neuronal excitation enhances odor discrimination.Current biology : CB · 2025Article
- A humanized Gs-coupled DREADD for circuit and behavior modulation.Frontiers in cellular neuroscience · 2025Article
- Acetylcholine and choline in honey bee (Apis mellifera) worker brood food are seasonal and age-dependent.Scientific reports · 2024Article
- Functional characterization of three G protein-coupled acetylcholine receptors in parasitic nematode Trichinella spiralis.International journal for parasitology. Drugs and drug resistance · 2023Article
- A Neural Circuit Controlling Virgin Female Aggression Induced by Mating-related Cues in Drosophila.Neuroscience bulletin · 2023Article
- Polygenic adaptation to overnutrition reveals a role for cholinergic signaling in longevity.bioRxiv : the preprint server for biology · 2023Article
- Muscarinic acetylcholine receptor activation synergizes the knockdown and toxicity of GABA-gated chloride channel insecticides.Pest management science · 2022Article
- Lateral axonal modulation is required for stimulus-specific olfactory conditioning in Drosophila.Current biology : CB · 2022Article
- Pirenzepine Binding Sites in the Brain of the HoneybeeInsects · 2022Article
- In Search of Synergistic Insect Repellents: Modeling of Muscarinic GPCR Interactions with Classical and Bitopic Photoactive Ligands.Molecules (Basel, Switzerland) · 2022Article
- Localization of muscarinic acetylcholine receptor-dependent rhythm-generating modules in theJournal of neurophysiology · 2022Article
- Transcriptional analysis of Bemisia tabaci MEAM1 cryptic species under the selection pressure of neonicotinoids imidacloprid, acetamiprid and thiamethoxam.BMC genomics · 2022Article
- The Neuromodulatory Basis of Aggression: Lessons From the Humble Fruit Fly.Frontiers in behavioral neuroscience · 2022Review
- GPCR voltage dependence controls neuronal plasticity and behavior.Nature communications · 2021Article
- Five Breakthroughs: A First Approximation of Brain Evolution From Early Bilaterians to Humans.Frontiers in neuroanatomy · 2021Article
- Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation.International journal for parasitology. Drugs and drug resistance · 2020Article
- Three-dimensional analysis of the heart function and effect cholinergic agonists in the cockroach Gromphadorhina portentosa.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2020Article
- Characterization of an A-Type Muscarinic Acetylcholine Receptor and Its Possible Non-neuronal Role in the Oriental Armyworm,Frontiers in physiology · 2020Article
- The Neurotransmitters Involved inFrontiers in behavioral neuroscience · 2020Review
Corrections and comments
- Erratum issuedde Valdivia, Ernesto Gonzalez [corrected to Gonzalez de Valdivia, Ernesto]
Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muscarinic acetylcholine receptors (mAChRs) play a central role in the mammalian nervous system. These receptors are G protein-coupled receptors (GPCRs), which are activated by the agonists acetylcholine and muscarine, and blocked by a variety of antagonists. Mammals have five mAChRs (m1-m5). In this study, we cloned two structurally related GPCRs from the fruit fly Drosophila melanogaster, which, after expression in Chinese hamster ovary cells, proved to be muscarinic acetylcholine receptors. One mAChR (the A-type; encoded by gene CG4356) is activated by acetylcholine (EC50, 5 × 10(-8) M) and muscarine (EC50, 6 × 10(-8) M) and blocked by the classical mAChR antagonists atropine, scopolamine, and 3-quinuclidinyl-benzilate (QNB), while the other (the B-type; encoded by gene CG7918) is also activated by acetylcholine, but has a 1,000-fold lower sensitivity to muscarine, and is not blocked by the antagonists. A- and B-type mAChRs were also cloned and functionally characterized from the red flour beetle Tribolium castaneum. Recently, Haga et al. (Nature 2012, 482: 547-551) published the crystal structure of the human m2 mAChR, revealing 14 amino acid residues forming the binding pocket for QNB. These residues are identical between the human m2 and the D. melanogaster and T. castaneum A-type mAChRs, while many of them are different between the human m2 and the B-type receptors. Using bioinformatics, one orthologue of the A-type and one of the B-type mAChRs could also be found in all other arthropods with a sequenced genome. Protostomes, such as arthropods, and deuterostomes, such as mammals and other vertebrates, belong to two evolutionarily distinct lineages of animal evolution that split about 700 million years ago. We found that animals that originated before this split, such as cnidarians (Hydra), had two A-type mAChRs. From these data we propose a model for the evolution of mAChRs.
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