ArticleJournal of bacteriology2013
Transcriptional regulation and characteristics of a novel N-acetylmuramoyl-L-alanine amidase gene involved in Bacillus thuringiensis mother cell lysis.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- MclX modulates the process of mother cell lysis by SpoIIID inApplied and environmental microbiology · 2026Article
- A Novel Enterococcus Phage Endolysin Lys22 with a Wide Host Range Against Mixed Biofilm ofPathogens (Basel, Switzerland) · 2025Article
- Characterization of a novel cell wall hydrolase CwlE involved inFrontiers in microbiology · 2023Article
- Identification of Genetic Markers for the Detection ofFoods (Basel, Switzerland) · 2022Article
- Expression of the Bacillus thuringiensisMicrobiology spectrum · 2022Article
- Deletion of the novel gene mother cell lysis X results in Cry1Ac encapsulation in theFrontiers in microbiology · 2022Article
- Identification and Functional Characterization of Two Homologous SpoVS Proteins Involved in Sporulation of Bacillus thuringiensis.Microbiology spectrum · 2021Article
- Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18.mSphere · 2020Article
- AToxins · 2020Article
- Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.Frontiers in microbiology · 2019Review
- Novel Cell Wall Hydrolase CwlC from Bacillus thuringiensis Is Essential for Mother Cell Lysis.Applied and environmental microbiology · 2018Article
- Disruption of Two-component System LytSR Affects Forespore Engulfment inFrontiers in cellular and infection microbiology · 2017Article
- Bacillus thuringiensis peptidoglycan hydrolase SleB171 involved in daughter cell separation during cell division.Acta biochimica et biophysica Sinica · 2016Article
- The Regulation of Exosporium-Related Genes in Bacillus thuringiensis.Scientific reports · 2016Article
- Transcription of the lysine-2,3-aminomutase gene in the kam locus of Bacillus thuringiensis subsp. kurstaki HD73 is controlled by both σ54 and σK factors.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5' rapid amplification of cDNA ends (5'-RACE)-PCR and transcriptional fusions with the lacZ gene indicated that transcription of the operon was directed by a promoter, P(cwlA), which is located upstream from the cwlA gene and that the transcription start site is a single 5'-end nucleotide residue T located 25 nucleotides (bp) upstream from the cwlA translational start codon. Moreover, the activity of P(cwlA) was controlled by σ(K). Morphological analysis suggested that the mutation of cwlB could delay spore release compared to the timing of spore release in the wild-type strain. Western blot assay demonstrated that purified CwlB bound to the B. thuringiensis cell wall. Observations with laser confocal microscopy and a green fluorescent protein-based reporter system demonstrated that the CwlB protein localizes to the cell envelope. All results suggest that the CwlB protein is involved in mother cell lysis in B. thuringiensis.
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