ArticleApplied and environmental microbiology2013
A novel muconic acid biosynthesis approach by shunting tryptophan biosynthesis via anthranilate.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 93 citations in OpenAlex.
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- Functional Analysis of a Salicylate Hydroxylase inJournal of fungi (Basel, Switzerland) · 2023Article
- EngineeredFrontiers in microbiology · 2023Article
- Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering.Nature communications · 2022Article
- Metabolic Engineering of Shikimic Acid Biosynthesis Pathway for the Production of Shikimic Acid and Its Branched Products in Microorganisms: Advances and Prospects.Molecules (Basel, Switzerland) · 2022Review
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- Discovery and Functional Analysis of a Salicylic Acid Hydroxylase from Aspergillus niger.Applied and environmental microbiology · 2021Article
- Engineering of Saccharomyces cerevisiae for anthranilate and methyl anthranilate production.Microbial cell factories · 2021Article
- Recent Advances in Microbial Production ofBiomolecules · 2020Review
- Fully biological production of adipic acid analogs from branched catechols.Scientific reports · 2020Article
- Improvement ofACS synthetic biology · 2020Article
- Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production.Metabolic engineering · 2019Article
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
- Biosensor-guided improvements in salicylate production by recombinant Escherichia coli.Microbial cell factories · 2019Article
- Cell Factory Design and Culture Process Optimization for Dehydroshikimate Biosynthesis inFrontiers in bioengineering and biotechnology · 2019Article
- Corynebacterium Cell Factory Design and Culture Process Optimization for Muconic Acid Biosynthesis.Scientific reports · 2018Article
- Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis.Nature communications · 2018Article
- Towards a sustainable bio-based economy: Redirecting primary metabolism to new products with plant synthetic biology.Plant science : an international journal of experimental plant biology · 2018Review
- Metabolic Engineering of the Shikimate Pathway for Production of Aromatics and Derived Compounds-Present and Future Strain Construction Strategies.Frontiers in bioengineering and biotechnology · 2018Article
- Metabolic engineering of a novel muconic acid biosynthesis pathway via 4-hydroxybenzoic acid in Escherichia coli.Applied and environmental microbiology · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Muconic acid is the synthetic precursor of adipic acid, and the latter is an important platform chemical that can be used for the production of nylon-6,6 and polyurethane. Currently, the production of adipic acid relies mainly on chemical processes utilizing petrochemicals, such as benzene, which are generally considered environmentally unfriendly and nonrenewable, as starting materials. Microbial synthesis from renewable carbon sources provides a promising alternative under the circumstance of petroleum depletion and environment deterioration. Here we devised a novel artificial pathway in Escherichia coli for the biosynthesis of muconic acid, in which anthranilate, the first intermediate in the tryptophan biosynthetic branch, was converted to catechol and muconic acid by anthranilate 1,2-dioxygenase (ADO) and catechol 1,2-dioxygenase (CDO), sequentially and respectively. First, screening for efficient ADO and CDO from different microbial species enabled the production of gram-per-liter level muconic acid from supplemented anthranilate in 5 h. To further achieve the biosynthesis of muconic acid from simple carbon sources, anthranilate overproducers were constructed by overexpressing the key enzymes in the shikimate pathway and blocking tryptophan biosynthesis. In addition, we found that introduction of a strengthened glutamine regeneration system by overexpressing glutamine synthase significantly improved anthranilate production. Finally, the engineered E. coli strain carrying the full pathway produced 389.96 ± 12.46 mg/liter muconic acid from simple carbon sources in shake flask experiments, a result which demonstrates scale-up potential for microbial production of muconic acid.
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