ArticleParasites & vectors2013
Identification of Trypanosoma cruzi discrete typing units (DTUs) through the implementation of a high-resolution melting (HRM) genotyping assay.
Article in Parasites & vectors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2020Pooled it
- Next-Generation Vaccines Against Neglected Diseases: New Promises from Genetically Modified Live-Attenuated Parasites and RNA Vaccines.Microorganisms · 2026Review
- Mechanistic Exploration ofPharmaceuticals (Basel, Switzerland) · 2026Article
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Diversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence ofMicroorganisms · 2024Article
- Evaluation of Molecular Methods to Identify Chagas Disease and Leishmaniasis in Blood Donation Candidates in Two Brazilian Centers.Pathogens (Basel, Switzerland) · 2023Article
- Editorial: Chagas disease novel drug targets and treatments.Frontiers in cellular and infection microbiology · 2023Article
- Consensus Enolase ofLife (Basel, Switzerland) · 2022Article
- A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR.PLoS neglected tropical diseases · 2020Article
- Identification of Phenylpyrazolone Dimers as a New Class of Anti-Trypanosoma cruzi Agents.ChemMedChem · 2019Article
- High-Resolution Melting Analysis as an Appropriate Method to Differentiate betweenIranian journal of public health · 2019Article
- The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi.Scientific reports · 2018Article
- Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection.PLoS neglected tropical diseases · 2017Article
- Geographical, landscape and host associations of Trypanosoma cruzi DTUs and lineages.Parasites & vectors · 2016Article
- Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis.Parasitology research · 2015Article
- Between a bug and a hard place: Trypanosoma cruzi genetic diversity and the clinical outcomes of Chagas disease.Expert review of anti-infective therapy · 2015Review
- Inflammatory responses and intestinal injury development during acute Trypanosoma cruzi infection are associated with the parasite load.Parasites & vectors · 2015Article
- Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.Parasites & vectors · 2014Article
- In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection.Parasites & vectors · 2014Article
- A DTU-dependent blood parasitism and a DTU-independent tissue parasitism during mixed infection of Trypanosoma cruzi in immunosuppressed mice.Parasitology research · 2014Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChagas disease, caused by Trypanosoma cruzi, is a geographically widespread anthropozoonosis that is considered a major public health problem in Latin America. Because this parasite presents high genetic variability, a nomenclature has been adopted to classify the parasite into six discrete typing units (DTUs): TcI, TcII, TcIII, TcIV, TcV, and TcVI, which present different eco-epidemiological, clinical, and geographic associations. Currently, the available genotyping methods present a series of drawbacks that implies the need for developing new methods for characterizing T. cruzi DTU's. The aim of this work was to genotype reference populations from T. cruzi by means of a High-Resolution Melting (HRM) genotyping assay.
findingsAmplification of the mini-exon gene allowed the genotyping of three distinct groups: TcI, TcII- TcIV- TcV, and TcIII-TcVI, while amplification of the 24sα gene generated non-overlapping melting temperature ranges for each DTU that were used to identify the groups in the six existing DTUs of Trypanosoma cruzi.
conclusionsThe proposed genotyping assay allowed discrimination of the six genetic groups by obtaining specific melting curves for each DTU. The application of this technique is proposed because of its specificity, sensitivity, high performance, and low cost compared with other previously described characterization methods.
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