ArticleBMC cell biology2013
Constant or fluctuating hyperglycemias increases cytomembrane stiffness of human umbilical vein endothelial cells in culture: roles of cytoskeletal rearrangement and nitric oxide synthesis.
Article in BMC cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Reduced cofilin activity as a mechanism contributing to endothelial cell stiffening in type 2 diabetes.American journal of physiology. Heart and circulatory physiology · 2025Article
- The endothelial plasma membrane lipidome and its remodeling under hyperglycemia: an exploratory study.Frontiers in molecular biosciences · 2025Article
- Endothelial cell serum and glucocorticoid regulated kinase 1 (SGK1) mediates vascular stiffening.Metabolism: clinical and experimental · 2024Article
- Bilayer vascular grafts with on-demand NO and HBioactive materials · 2024Article
- Advanced Glycation End Products Effects on Adipocyte Niche Stiffness and Cell Signaling.International journal of molecular sciences · 2023Article
- Modelling Hyperglycaemia in an Epithelial Membrane Model: Biophysical Characterisation.Biomolecules · 2022Article
- Therapeutic hypothermia augments the restorative effects of PKC-β and Nox2 inhibition on an in vitro model of human blood-brain barrier.Metabolic brain disease · 2021Article
- Functional differences between healthy and diabetic endothelial cells on topographical cues.Biomaterials · 2018Article
- Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation.Mediators of inflammation · 2017Article
- Low-level laser irradiation effect on endothelial cells under conditions of hyperglycemia.Lasers in medical science · 2016Article
- High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy.Diabetology & metabolic syndrome · 2015Article
- Hyperglycemia-Induced Changes in Hyaluronan Contribute to Impaired Skin Wound Healing in Diabetes: Review and Perspective.International journal of cell biology · 2015Review
- Aberrant phenotype in human endothelial cells of diabetic origin: implications for saphenous vein graft failure?Journal of diabetes research · 2015Article
- Stiffness memory of EA.hy926 endothelial cells in response to chronic hyperglycemia.Cardiovascular diabetology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious studies have implicated continuous or intermittent hyperglycemia in altered endothelium-derived nitric oxide (NO) synthesis. NO can regulate both the F-actin cytoskeleton and endothelial cell membrane stiffness. Atomic force microscopy (AFM) is a powerful tool that can be used to study plasma membrane deformability at the single cell level. As membrane stiffness is partially dependent on filamentous F-actin, the interdependence of these parameters can be studied through the combined approaches of AFM and laser scanning confocal microscopy (LSCM). In the present study, we evaluated the effects of constant or fluctuating hyperglycemia on endothelial-derived NO synthesis, the cytoskeletal contribution and endothelial cell membrane stiffness.
resultsCompared to control cells cultured in low glucose (5 mM), constant (25 mM) or fluctuating (25/5 mM) high glucose significantly decreased NO release along with stiffening of endothelial cell membranes and F-actin rearrangement. The non-selective nitric oxide synthase (NOS) inhibitor, N(G)-nitro-(L)-arginine methyl ester ((L)-NAME) exerted similar effects on endothelial cells. Increasing concentrations of (L)-NAME (from 0.1 to 1 mM) exacerbated these effects in a concentration-dependent manner.
conclusionsResult from the present study suggest that stiffening endothelial cell membranes are associated with decreased NO synthesis, which was established through the F-actin cytoskeletal redistribution. The precise mechanisms of hyperglycemia-induced endothelial dysfunction require further investigation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.