ArticleJournal of the American Chemical Society2013
A catalyCEST MRI contrast agent that detects the enzyme-catalyzed creation of a covalent bond.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- Peptide-Based pH-Responsive MRI-CEST Agents: In Vivo Comparison between a Selected Pentapeptide and the Established Iopamidol Reference in Tumor pH Mapping Ability.Chemical & biomedical imaging · 2026Article
- Photoactivatable, Biomimetic Ligand photoMultiTASQ Traps DNA/RNA G‑Quadruplexes and Their Protein Binding Partners.ACS bio & med chem Au · 2025Article
- Review
- Protein and peptide engineering for chemical exchange saturation transfer imaging in the age of synthetic biology.NMR in biomedicine · 2023Review
- Detection of tissue pH with quantitative chemical exchange saturation transfer magnetic resonance imaging.NMR in biomedicine · 2023Review
- Simultaneous Evaluations of pH and Enzyme Activity with a CEST MRI Contrast Agent.ACS sensors · 2021Article
- Probing Reversible Guest Binding with HyperpolarizedMolecules (Basel, Switzerland) · 2020Review
- Assessments of tumor metabolism with CEST MRI.NMR in biomedicine · 2019Review
- Detecting acid phosphatase enzymatic activity with phenol as a chemical exchange saturation transfer magnetic resonance imaging contrast agent (PhenolCEST MRI).Biosensors & bioelectronics · 2019Article
- Chemistry of MRI Contrast Agents: Current Challenges and New Frontiers.Chemical reviews · 2019Review
- Triazoles as TChemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Detection of Enzyme Activity and Inhibition during Studies in Solution, In Vitro and In Vivo with CatalyCEST MRI.Molecular imaging and biology · 2018Article
- The diamond anniversary of tissue transglutaminase: a protein of many talents.Drug discovery today · 2018Review
- Clinical applications of chemical exchange saturation transfer (CEST) MRI.Journal of magnetic resonance imaging : JMRI · 2018Review
- Detecting in vivo urokinase plasminogen activator activity with a catalyCEST MRI contrast agent.NMR in biomedicine · 2017Article
- Noninvasive detection of enzyme activity in tumor models of human ovarian cancer using catalyCEST MRI.Magnetic resonance in medicine · 2017Article
- A biomarker-responsive TMagnetic resonance in medicine · 2017Article
- Functional nanoparticles for magnetic resonance imaging.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2016Review
- Diamagnetic Imaging Agents with a Modular Chemical Design for Quantitative Detection of β-Galactosidase and β-Glucuronidase Activities with CatalyCEST MRI.Bioconjugate chemistry · 2016Article
- Advances in Magnetic Resonance Imaging Contrast Agents for Biomarker Detection.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
CatalyCEST MRI can detect enzyme activity by employing contrast agents that are detected through chemical exchange saturation transfer (CEST). A CEST agent, Tm-DO3A-cadaverine, has been designed to detect the catalytic activity of transglutaminase (TGase), which creates a covalent bond between the agent and the side chain of a glutamine amino acid residue. CEST appeared at -9.2 ppm after TGase conjugated Tm-DO3A-cadaverine to albumin, which also caused a decrease in CEST from albumin at +4.6 ppm. Studies with model peptides revealed similar appearances and decreases in detectable CEST effects following TGase-catalyzed conjugation of the contrast agent and peptide. The MR frequencies and amplitudes of these CEST effects were dependent on the peptide sequence, which demonstrated the sensitivity of CEST agents to ligand conformations that may be exploited to create more responsive molecular imaging agents. The chemical exchange rates of the substrates and conjugated products were measured by fitting modified Bloch equations to CEST spectra, which demonstrated that changes in exchange rates can also be used to detect the formation of a covalent bond by catalyCEST MRI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.