ArticleMolecular microbiology2013
Architecture and assembly of the Gram-positive cell wall.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 136 citations in OpenAlex.
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- Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis.Nature communications · 2025Article
- Revealing the Response Mechanism ofFoods (Basel, Switzerland) · 2025Article
- Fungal β-Glucans Enhance Lactic Acid Bacteria Growth by Shortening Their Lag Phase and Increasing Growth Rate.Microorganisms · 2025Article
- Visualization of peptidoglycan layer isolated from gliding diderm bacteria,Biophysics and physicobiology · 2025Article
- Thicket and Mesh: How the Outer Membrane Can Resist Tension Imposed by the Cell Wall.The journal of physical chemistry. B · 2024Article
- The choline-binding proteins PspA, PspC, and LytA ofInfection and immunity · 2023Article
- Methacrylate Cationic Nanoparticles Activity against Different Gram-Positive Bacteria.Antibiotics (Basel, Switzerland) · 2023Article
- Class A Penicillin-Binding Protein C Is Responsible for Stress Response by Regulation of Peptidoglycan Assembly in Clavibacter michiganensis.Microbiology spectrum · 2022Article
- Influence of NaCl and pH on lysostaphin catalytic activity, cell binding, and bacteriolytic activity.Applied microbiology and biotechnology · 2022Article
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- Antimicrobial activities and phytochemical properties ofJournal of medicine and life · 2022Article
- Inhibition ofMicrobiology (Reading, England) · 2022Article
- What have molecular simulations contributed to understanding of Gram-negative bacterial cell envelopes?Microbiology (Reading, England) · 2022Review
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- Influences of geochemical factors and substrate availability on Gram-positive and Gram-negative bacterial distribution and bio-processes in ageing municipal landfills.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Molecular Machinery Responsible for Graphene Oxide's Distinct Inhibitory Effects towardsACS applied bio materials · 2021Article
- An updated toolkit for exploring bacterial cell wall structure and dynamics.Faculty reviews · 2021Review
- How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure.Frontiers in microbiology · 2021Article
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4 authors at 4 institutions in 1 country.
Funding
Abstract
The bacterial cell wall is a mesh polymer of peptidoglycan--linear glycan strands cross-linked by flexible peptides--that determines cell shape and provides physical protection. While the glycan strands in thin 'Gram-negative' peptidoglycan are known to run circumferentially around the cell, the architecture of the thicker 'Gram-positive' form remains unclear. Using electron cryotomography, here we show that Bacillus subtilis peptidoglycan is a uniformly dense layer with a textured surface. We further show it rips circumferentially, curls and thickens at free edges, and extends longitudinally when denatured. Molecular dynamics simulations show that only atomic models based on the circumferential topology recapitulate the observed curling and thickening, in support of an 'inside-to-outside' assembly process. We conclude that instead of being perpendicular to the cell surface or wrapped in coiled cables (two alternative models), the glycan strands in Gram-positive cell walls run circumferentially around the cell just as they do in Gram-negative cells. Together with providing insights into the architecture of the ultimate determinant of cell shape, this study is important because Gram-positive peptidoglycan is an antibiotic target crucial to the viability of several important rod-shaped pathogens including Bacillus anthracis, Listeria monocytogenes, and Clostridium difficile.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.