ArticleMonoclonal antibodies in immunodiagnosis and immunotherapy2013
Expression and Purification of the scFv from hybridoma cells secreting a monoclonal antibody against S PROTEIN of PEDV.
Article in Monoclonal antibodies in immunodiagnosis and immunotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Construction, expression, and characterization of scFv fragment against Fasciola gigantica cathepsin L1H.Parasitology research · 2025Article
- Research progress of porcine epidemic diarrhea virus S protein.Frontiers in microbiology · 2024Review
- Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa.Planta medica · 2017Article
- A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.The Journal of veterinary medical science · 2016Article
- Optimized Expression, Purification of Herpes B Virus gD Protein in Escherichia coli, and Production of Its Monoclonal Antibodies.Jundishapur journal of microbiology · 2016Article
- A novel anti-p21Ras scFv antibody reacting specifically with human tumour cell lines and primary tumour tissues.BMC cancer · 2016Article
- Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique.Journal of virological methods · 2015Article
- Preparation of monoclonal antibodies against bovine haptoglobin.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Article
- A polyclonal antibody against recombinant bovine haptoglobin expressed in Escherichia coli.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2013Article
- Screening and antiviral analysis of phages that display peptides with an affinity to subunit C of porcine aminopeptidase.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The variable regions of the heavy chain (VH) and light chain (VL) were amplified by RT-PCR from the hybridoma 6E6, which secretes the monoclonal antibody against PEDV S protein. The VL and VH amplicons were combined using SOE-PCR by a 12 amino acid flexible linker (SSGGGGSGGGGS), which produced the scFv gene (named scFv/6E6). After sequence analysis, the scFv/6E6 gene was cloned into the prokaryotic expression vector pGEX-6p-1 with a GST-tag. The recombinant scFv/6E6 protein was successfully expressed in recombinant Escherichia coli by IPTG induction. Moreover, the recombinant scFv/6E6 protein was purified from the inclusion body form by the gel-cutting measure followed by electroelution and dialysis. The recombinant scFv/6E6 protein reported here will provide some basis for further antiviral drug research based on the scFv molecule.
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