ArticleACS chemical neuroscience2013
Kinetics of the dopamine transporter in Drosophila larva.
Article in ACS chemical neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- MicroRNA-132/212 negatively modulates opioid reward by targeting dopamine transporter in the ventral tegmental area.Translational psychiatry · 2026Article
- Fast-Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body.Angewandte Chemie (International ed. in English) · 2022Article
- Effectiveness and Relationship between Biased and Unbiased Measures of Dopamine Release and Clearance.ACS chemical neuroscience · 2022Article
- Ring Finger Protein 11 (RNF11) Modulates Dopamine Release in Drosophila.Neuroscience · 2021Article
- How Important Is the Use of Cocaine and Amphetamines in the Development of Parkinson Disease? A Computational Study.Neurotoxicity research · 2020Article
- Dop1R1, a type 1 dopaminergic receptor expressed in Mushroom Bodies, modulates Drosophila larval locomotion.PloS one · 2020Article
- Model systems for analysis of dopamine transporter function and regulation.Neurochemistry international · 2019Review
- Dopamine release in mushroom bodies of the honey bee (Apis mellifera L.) in response to aversive stimulation.Scientific reports · 2018Article
- Electrochemical Measurements of Acetylcholine-Stimulated Dopamine Release in Adult Drosophila melanogaster Brains.Analytical chemistry · 2018Article
- Drosophila as a Model System for Neurotransmitter Measurements.ACS chemical neuroscience · 2018Review
- Nicotinic acetylcholine receptor (nAChR) mediated dopamine release in larval Drosophila melanogaster.Neurochemistry international · 2018Article
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- Fast-Scan Cyclic Voltammetry (FSCV) Detection of Endogenous Octopamine in Drosophila melanogaster Ventral Nerve Cord.ACS chemical neuroscience · 2016Article
- Comparison of dopamine kinetics in the larval Drosophila ventral nerve cord and protocerebrum with improved optogenetic stimulation.Journal of neurochemistry · 2015Article
- Characterization of dopamine releasable and reserve pools in Drosophila larvae using ATP/P2X2 -mediated stimulation.Journal of neurochemistry · 2015Article
- Optogenetic control of serotonin and dopamine release in Drosophila larvae.ACS chemical neuroscience · 2014Article
- Drosophila melanogaster as a genetic model system to study neurotransmitter transporters.Neurochemistry international · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Dopamine transporters (DAT) regulate neurotransmission and are important in diseases such as addiction and attention deficit hyperactivity disorder. The Drosophila dopamine transporter (dDAT) is analogous to the mammalian DAT, but Michaelis-Menten kinetic parameters have not been characterized in vivo. In this study, dopamine clearance kinetics were measured in a Drosophila larval CNS using an implanted carbon-fiber microelectrode and fast-scan cyclic voltammetry. Dopamine was pressure ejected from a micropipet implanted 15-20 μm from the microelectrode. Clearance of exogenously applied dopamine was significantly reduced in dDAT null (fumin) mutants, and kinetic constants in these mutants were used to determine clearance by other mechanisms including diffusion. After correction for diffusion, the maximal rate of uptake, Vmax, was estimated to be 0.11 ± 0.02 μM/s and Km was 1.3 ± 0.6 μM in wild-type flies. The clearance rate was significantly reduced following treatment with the DAT inhibitor cocaine in wild-type flies, but not in fumin mutants, which indicates that serotonin transporter is not contributing significantly to dopamine clearance in these larvae. Clearance of endogenous dopamine, evoked by optical stimulation in flies expressing Channelrhodopsin2, was similar to clearance of exogenous dopamine, but it was not possible to evoke concentrations that were close to saturation. The ability to quickly assess the role of the dopamine transporter in any Drosophila larva will be useful for future studies of how transporters regulate neurotransmission and to understand the underlying mechanisms of drug addiction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.