ArticleThe Journal of biological chemistry2013
Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Testicular Heat-Shock Protein Expression in Rats Following 3.5 GHz and 24 GHz RF-EMF Exposure.International journal of molecular sciences · 2026Article
- Article
- TPTE, a testis-specific PTEN family member, drives spermatogenesis via PI(4,5)PCell death & disease · 2026Article
- TSSKL is essential for sperm mitochondrial morphogenesis and male fertility in moths.PLoS genetics · 2025Article
- Long COVID-19 autoantibodies and their potential effect on fertility.Frontiers in immunology · 2025Article
- Loss-of-function in testis-specific serine/threonine protein kinase triggers male infertility in an invasive moth.Communications biology · 2024Article
- Retinoic acid loaded with chitosan nanoparticles improves spermatogenesis in scrotal hyperthermia in mice.Clinical and experimental reproductive medicine · 2023Article
- Multi-omics analysis reveals changes in tryptophan and cholesterol metabolism before and after sexual maturation in captive macaques.BMC genomics · 2023Article
- Identification of the TSSK4 Alternative Spliceosomes and Analysis of the Function of the TSSK4 Protein in Yak (Animals : an open access journal from MDPI · 2022Article
- TheFrontiers in molecular biosciences · 2022Article
- Human Hsp90 cochaperones: perspectives on tissue-specific expression and identification of cochaperones with similar in vivo functions.Cell stress & chaperones · 2021Review
- Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†.Biology of reproduction · 2020Review
- HIPK4 is essential for murine spermiogenesis.eLife · 2020Article
- A Long Non-coding RNA Lnc712 Regulates Breast Cancer Cell Proliferation.International journal of biological sciences · 2020Article
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- Article
- The regulation of heat shock proteins in response to dehydration in Xenopus laevis.Cell stress & chaperones · 2018Article
- Progesterone requires heat shock protein 90 (HSP90) in human sperm to regulate motility and acrosome reaction.Journal of assisted reproduction and genetics · 2017Article
- Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis.Molecular & cellular proteomics : MCP · 2016Article
- HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death.Cell death & disease · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermiogenesis is characterized by a profound morphological differentiation of the haploid spermatid into spermatozoa. The testis-specific serine/threonine kinases (TSSKs) comprise a family of post-meiotic kinases expressed in spermatids, are critical to spermiogenesis, and are required for male fertility in mammals. To explore the role of heat shock protein 90 (HSP90) in regulation of TSSKs, the stability and catalytic activity of epitope-tagged murine TSSKs were assessed in 293T and COS-7 cells. TSSK1, -2, -4, and -6 (small serine/threonine kinase) were all found to associate with HSP90, and pharmacological inhibition of HSP90 function using the highly specific drugs 17-AAG, SNX-5422, or NVP-AUY922 reduced TSSK protein levels in cells. The attenuation of HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation, indicating that HSP90 acts to control ubiquitin-mediated catabolism of the TSSKs. To study HSP90 and TSSKs in germ cells, a mouse primary spermatid culture model was developed and characterized. Using specific antibodies against murine TSSK2 and -6, it was demonstrated that HSP90 inhibition resulted in a marked decrease of the endogenous kinases in spermatids. Together, our findings demonstrate that HSP90 plays a broad and critical role in stabilization and activation of the TSSK family of protein kinases.
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Registered trials
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