ArticleNucleic acids research2013
Din7 and Mhr1 expression levels regulate double-strand-break-induced replication and recombination of mtDNA at ori5 in yeast.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number.Nucleic acids research · 2026Article
- Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae.Scientific reports · 2025Article
- Two mitochondrial HMG-box proteins, Cim1 and Abf2, antagonistically regulate mtDNA copy number in Saccharomyces cerevisiae.Nucleic acids research · 2023Article
- From Genome Variation to Molecular Mechanisms: What we Have Learned From Yeast Mitochondrial Genomes?Frontiers in microbiology · 2022Review
- Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.PLoS genetics · 2021Article
- Thermodynamic analysis of DNA hybridization signatures near mitochondrial DNA deletion breakpoints.iScience · 2021Article
- Review
- Prevention of mitochondrial genomic instability in yeast by the mitochondrial recombinase Mhr1.Scientific reports · 2019Article
- Saccharomyces cerevisiae Mhr1 can bind Xho I-induced mitochondrial DNA double-strand breaks in vivo.Mitochondrion · 2018Article
- Plant organellar DNA primase-helicase synthesizes RNA primers for organellar DNA polymerases using a unique recognition sequence.Nucleic acids research · 2017Article
- Regulation of Small Mitochondrial DNA Replicative Advantage by Ribonucleotide Reductase inG3 (Bethesda, Md.) · 2017Article
- Evidence for double-strand break mediated mitochondrial DNA replication in Saccharomyces cerevisiae.Nucleic acids research · 2017Article
- Review
- Mitochondria and oxidative stress in heart aging.Age (Dordrecht, Netherlands) · 2016Review
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- Article
- Crosstalk between mitochondrial stress signals regulates yeast chronological lifespan.Mechanisms of ageing and development · 2014Article
- Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA.Microbiology and molecular biology reviews : MMBR · 2013Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ntg1 and Mhr1 proteins initiate rolling-circle mitochondrial (mt) DNA replication to achieve homoplasmy, and they also induce homologous recombination to maintain mitochondrial genome integrity. Although replication and recombination profoundly influence mitochondrial inheritance, the regulatory mechanisms that determine the choice between these pathways remain unknown. In Saccharomyces cerevisiae, double-strand breaks (DSBs) introduced by Ntg1 at the mitochondrial replication origin ori5 induce homologous DNA pairing by Mhr1, and reactive oxygen species (ROS) enhance production of DSBs. Here, we show that a mitochondrial nuclease encoded by the nuclear gene DIN7 (DNA damage inducible gene) has 5'-exodeoxyribonuclease activity. Using a small ρ(-) mtDNA bearing ori5 (hypersuppressive; HS) as a model mtDNA, we revealed that DIN7 is required for ROS-enhanced mtDNA replication and recombination that are both induced at ori5. Din7 overproduction enhanced Mhr1-dependent mtDNA replication and increased the number of residual DSBs at ori5 in HS-ρ(-) cells and increased deletion mutagenesis at the ori5 region in ρ(+) cells. However, simultaneous overproduction of Mhr1 suppressed all of these phenotypes and enhanced homologous recombination. Our results suggest that after homologous pairing, the relative activity levels of Din7 and Mhr1 modulate the preference for replication versus homologous recombination to repair DSBs at ori5.
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