ArticleThe ISME journal2013
Deep sequencing of non-ribosomal peptide synthetases and polyketide synthases from the microbiomes of Australian marine sponges.
Article in The ISME journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Exploring the secrets of marine microorganisms: Unveiling secondary metabolites through metagenomics.Microbial biotechnology · 2024Review
- Non-ribosomal peptide synthase profiles remain structurally similar despite minimally shared features across fungus-farming termite microbiomes.ISME communications · 2024Article
- The natural product biosynthesis potential of the microbiomes of Earth - Bioprospecting for novel anti-microbial agents in the meta-omics era.Computational and structural biotechnology journal · 2022Review
- Microorganisms Associated with the Marine SpongeMicroorganisms · 2020Article
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- Diversity of Gene Clusters for Polyketide and Nonribosomal Peptide Biosynthesis Revealed by Metagenomic Analysis of the Yellow Sea Sediment.Frontiers in microbiology · 2018Article
- Recovering Genomics Clusters of Secondary Metabolites from Lakes Using Genome-Resolved Metagenomics.Frontiers in microbiology · 2018Article
- Bacterial natural product biosynthetic domain composition in soil correlates with changes in latitude on a continent-wide scale.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Antibacterial Activities of Bacteria Isolated from the Marine Sponges Isodictya compressa and Higginsia bidentifera Collected from Algoa Bay, South Africa.Marine drugs · 2017Article
- A computationally simplistic poly-phasic approach to explore microbial communities from the Yucatan aquifer as a potential sources of novel natural products.Journal of microbiology (Seoul, Korea) · 2016Article
- Discovery Strategies of Bioactive Compounds Synthesized by Nonribosomal Peptide Synthetases and Type-I Polyketide Synthases Derived from Marine Microbiomes.Marine drugs · 2016Review
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- Diversity of Natural Product Biosynthetic Genes in the Microbiome of the Deep Sea Sponges Inflatella pellicula, Poecillastra compressa, and Stelletta normani.Frontiers in microbiology · 2016Article
- Sample Processing Impacts the Viability and Cultivability of the Sponge Microbiome.Frontiers in microbiology · 2016Article
- Targeted metagenomics as a tool to tap into marine natural product diversity for the discovery and production of drug candidates.Frontiers in microbiology · 2015Review
- Filamentous fungi from extreme environments as a promising source of novel bioactive secondary metabolites.Frontiers in microbiology · 2015Article
- Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils.PloS one · 2015Article
- Article
- Mining the metabiome: identifying novel natural products from microbial communities.Chemistry & biology · 2014Review
- Metagenomic small molecule discovery methods.Current opinion in microbiology · 2014Article
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Authors and funding
5 authors.
Funding
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Abstract
The biosynthesis of non-ribosomal peptide and polyketide natural products is facilitated by multimodular enzymes that contain domains responsible for the sequential condensation of amino and carboxylic subunits. These conserved domains provide molecular targets for the discovery of natural products from microbial metagenomes. This study demonstrates the application of tag-encoded FLX amplicon pyrosequencing (TEFAP) targeting non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) genes as a method for determining the identity and diversity of natural product biosynthesis genes. To validate this approach, we assessed the diversity of NRPS and PKS genes within the microbiomes of six Australian marine sponge species using both TEFAP and metagenomic whole-genome shotgun sequencing approaches. The TEFAP approach identified 100 novel ketosynthase (KS) domain sequences and 400 novel condensation domain sequences within the microbiomes of the six sponges. The diversity of KS domains within the microbiome of a single sponge species Scopalina sp. exceeded that of any previously surveyed marine sponge. Furthermore, this study represented the first to target the condensation domain from NRPS biosynthesis and resulted in the identification of a novel condensation domain lineage. This study highlights the untapped potential of Australian marine sponges for the isolation of novel bioactive natural products. Furthermore, this study demonstrates that TEFAP approaches can be applied to functional genes, involved in natural product biosynthesis, as a tool to aid natural product discovery. It is envisaged that this approach will be used across multiple environments, offering an insight into the biological processes that influence the production of secondary metabolites.
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