ArticleNature protocols2013
Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
83 citing papers in PubMed, 141 citations in OpenAlex.
- No Female Germline Stem Cells Were Identified by Advanced Dual Recombinase-Based Lineage Tracing System.Cell proliferation · 2026Article
- Mouse Mesonephros Promotes Differentiation of Oogonial Stem Cells into Follicle-like Structures via the PI3K/AKT Signaling Pathway.International journal of molecular sciences · 2026Article
- Dual recombinases-mediated genetic tracing reveals no postnatal neo-oogenesis in mice.iScience · 2026Article
- Fertility preservation and subsequent reproductive outcomes in female pediatric patients with hematologic malignancies.Frontiers in endocrinology · 2026Review
- Maternal sleep deprivation during pregnancy induced offspring germ cells loss through ferroptosis.Cell death discovery · 2025Article
- Single-Cell Transcriptomic Analysis of the Potential Mechanisms of Follicular Development inInternational journal of molecular sciences · 2025Article
- Advances inFrontiers in endocrinology · 2025Review
- Ovarian microenvironment: challenges and opportunities in protecting against chemotherapy-associated ovarian damage.Human reproduction update · 2024Review
- Article
- Single-Cell Atlas of Human Ovaries Reveals The Role Of The Pyroptotic Macrophage in Ovarian Aging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Mechanisms of mitochondrial dysfunction in ovarian aging and potential interventions.Frontiers in endocrinology · 2024Review
- Innovative Strategies for Fertility Preservation in Female Cancer Survivors: New Hope from Artificial Ovary Construction and Stem Cell-Derived Neo-Folliculogenesis.Healthcare (Basel, Switzerland) · 2023Review
- In Reply: Revisiting Claims of the Continued Absence of Functional Germline Stem Cells in Adult Ovaries.Stem cells (Dayton, Ohio) · 2023Article
- The Continued Absence of Functional Germline Stem Cells in Adult Ovaries.Stem cells (Dayton, Ohio) · 2023Article
- Revisiting Claims of the Continued Absence of Functional Germline Stem Cells in Adult Ovaries.Stem cells (Dayton, Ohio) · 2023Article
- Identification of oogonial stem cells in chicken ovary.Cell proliferation · 2023Article
- Characterization of Oogonial Stem Cells in Adult Mouse Ovaries with Age and Comparison to In Silico Data on Human Ovarian Aging.Stem cells and development · 2023Article
- Mitochondrial Transfer into Human Oocytes Improved Embryo Quality and Clinical Outcomes in Recurrent Pregnancy Failure Cases.International journal of molecular sciences · 2023Article
- Ovarian aging: mechanisms and intervention strategies.Medical review (2021) · 2022Review
- Identification of Stem Cell-Like Cells in the Ovary.Tissue engineering and regenerative medicine · 2022Review
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Accruing evidence indicates that production of new oocytes (oogenesis) and their enclosure by somatic cells (folliculogenesis) are processes not limited to the perinatal period in mammals. Endpoints ranging from oocyte counts to genetic lineage tracing and transplantation experiments support a paradigm shift in reproductive biology involving active renewal of oocyte-containing follicles during postnatal life. The recent purification of mitotically active oocyte progenitor cells, termed female germline stem cells (fGSCs) or oogonial stem cells (OSCs), from mouse and human ovaries opens up new avenues for research into the biology and clinical utility of these cells. Here we detail methods for the isolation of mouse and human OSCs from adult ovarian tissue, cultivation of the cells after purification, and characterization of the cells before and after ex vivo expansion. The latter methods include analysis of germ cell-specific markers and in vitro oogenesis, as well as the use of intraovarian transplantation to test the oocyte-forming potential of OSCs in vivo.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.