ArticlePloS one2013
Effect of different culture systems and 3, 5, 3'-triiodothyronine/follicle-stimulating hormone on preantral follicle development in mice.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Effect of metformin treatment on ovarian function in hypothyroid rats.Open veterinary journal · 2026Article
- Role of TInternational journal of molecular sciences · 2025Article
- Associations between a normal-range free thyroxine concentration and ovarian reserve in infertile women undergoing treatment via assisted reproductive technology.Reproductive biology and endocrinology : RB&E · 2024Article
- Prenatal and postnatal exposure to polychlorinated biphenyls alter follicle numbers, gene expression, and a proliferation marker in the rat ovary.Reproductive toxicology (Elmsford, N.Y.) · 2023Article
- The effects and mechanism of taxanes on chemotherapy-associated ovarian damage: A review of current evidence.Frontiers in endocrinology · 2022Review
- The relationship between thyroid function and ovarian reserve: a prospective cross-sectional study.Thyroid research · 2021Article
- Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles.Journal of ovarian research · 2020Article
- Thyroid hormones T3 and T4 regulate human luteinized granulosa cells, counteracting apoptosis and promoting cell survival.Journal of endocrinological investigation · 2020Article
- Thyroid Hormones and Functional Ovarian Reserve: Systemic vs. Peripheral Dysfunctions.Journal of clinical medicine · 2020Review
- Article
- Thyroid hormones act as mitogenic and pro survival factors in rat ovarian follicles.Journal of endocrinological investigation · 2019Article
- Mitochondrial Protein Turnover Is Critical for Granulosa Cell Proliferation and Differentiation in Antral Follicles.Journal of the Endocrine Society · 2019Article
- Role of OCT4 in the Regulation of FSH-Induced Granulosa Cells Growth in Female Mice.Frontiers in endocrinology · 2019Article
- Association of Thyroid Function and Autoimmunity with Ovarian Reserve in Women Seeking Infertility Care.Thyroid : official journal of the American Thyroid Association · 2018Article
- Elevated serum thyroid-stimulating hormone is associated with decreased anti-Müllerian hormone in infertile women of reproductive age.Journal of assisted reproduction and genetics · 2015Article
- Effect of azaline B on follicular development and functions in the hamster.Molecular and cellular endocrinology · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanical method to isolate preantral follicle has been reported for many years. However, the culture systems in vitro are still unstable. The aim of this study was to analyze the effect of the culture system of mice preantral follicles on the follicular development in vitro. The results showed that the 96-well plate system was the most effective method for mice follicle development in vitro (volume change: 51.71%; survival rate: 89%, at day 4). Follicle-stimulating hormone (FSH) and Thyroid hormone (TH) are important for normal follicular development and dysregulation of hormones are related with impaired follicular development. To determine the effect of hormone on preantral follicular development, we cultured follicle with hormones in the 96-well plate culture system and found that FSH significantly increased preantral follicular growth on day 4. The FSH-induced growth action was markedly enhanced by T₃ although T₃ was ineffective alone. We also demonstrated by QRT-PCR that T₃ significantly enhanced FSH-induced up-regulation of Xiap mRNA level. Meanwhile, Bad, cell death inducer, was markedly down-regulated by the combination of hormones. Moreover, QRT-PCR results were also consistent with protein regulation which detected by Western Blotting analysis. Taken together, the findings of the present study demonstrate that 96-well plate system is an effective method for preantral follicle development in vitro. Moreover, these results provide insights on the role of thyroid hormone in increasing FSH-induced preantral follicular development, which mediated by up-regulating Xiap and down-regulating Bad.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.