ArticleJournal of clinical microbiology2013
Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Bismuth-Based Quadruple Therapy as First-Line Treatment for Clarithromycin-ResistantGut and liver · 2024Trial
- Trial
- CurrentJGH open : an open access journal of gastroenterology and hepatology · 2026Review
- Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Comparative analysis of the detection of antibiotic genotypic resistance with gastric mucosa, gastric fluid, and fecal samples in patients withJournal of clinical microbiology · 2025Article
- Ecology ofBiofilm · 2024Review
- Molecular tools of antibiotic resistance forFrontiers in gastroenterology (Lausanne, Switzerland) · 2024Review
- Real-Time Polymerase Chain Reaction for the Detection ofGut and liver · 2023Article
- Correlations between Endoscopic and Histopathological Assessment ofLife (Basel, Switzerland) · 2022Article
- Ultrasensitive detection of β-lactamase-associated drug-resistant bacteria using a novel mass-tagged probe-mediated cascaded signal amplification strategy.Chemical science · 2022Article
- Development of a Novel Peptide Nucleic Acid Probe for the Detection ofMicroorganisms · 2022Article
- Traditional and Modern Diagnostic Approaches in Diagnosing PediatricChildren (Basel, Switzerland) · 2022Review
- Evolution of Diagnostic Methods forDiagnostics (Basel, Switzerland) · 2022Review
- Microfluidics combined with fluorescenceFrontiers in bioengineering and biotechnology · 2022Article
- Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Trends inMicrobial genomics · 2020Review
- Clinical Utility of PNA-FISH for Burn Wound Diagnostics: A Noninvasive, Culture-Independent Technique for Rapid Identification of Pathogenic Organisms in Burn Wounds.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- Peptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance inGut and liver · 2018Article
- Diagnosis of Helicobacter pylori infection : A short review.Wiener klinische Wochenschrift · 2018Review
- Rapid identification ofThe journal of pathology. Clinical research · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori clarithromycin resistance. In the retrospective study (n = 30 patients), full agreement between PNA-FISH and PCR-sequencing was observed. Compared to the reference method (culture followed by Etest), the specificity and sensitivity of PNA-FISH were 90.9% (95% confidence interval [CI], 57.1% to 99.5%) and 84.2% (95% CI, 59.5% to 95.8%), respectively. In the prospective cohort (n = 93 patients), 21 cases were positive by culture. For the patients harboring clarithromycin-resistant H. pylori, the method showed sensitivity of 80.0% (95% CI, 29.9% to 98.9%) and specificity of 93.8% (95% CI, 67.7% to 99.7%). These values likely represent underestimations, as some of the discrepant results corresponded to patients infected by more than one strain. PNA-FISH appears to be a simple, quick, and accurate method for detecting H. pylori clarithromycin resistance in paraffin-embedded biopsy specimens. It is also the only one of the methods assessed here that allows direct and specific visualization of this microorganism within the biopsy specimens, a characteristic that allowed the observation that cells of different H. pylori strains can subsist in very close proximity in the stomach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.