ArticleJournal of assisted reproduction and genetics2013
Chromosomal integrity of human preimplantation embryos at different days post fertilization.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Mapping of long interspersed element-1 (L1) insertions by TIPseq provides information about sub chromosomal genetic variation in human embryos.Journal of assisted reproduction and genetics · 2024Article
- Control of LINE-1 Expression Maintains Genome Integrity in Germline and Early Embryo Development.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Improving the clinical outcomes by extended culture of day 3 embryos with low blastomere number to blastocyst stage following frozen-thawed embryo transfer.Archives of gynecology and obstetrics · 2021Article
- The Effect of Teratozoospermia on Sex Chromosomes in Human Embryos.The application of clinical genetics · 2021Article
- Developmental potential of clinically discarded human embryos and associated chromosomal analysis.Scientific reports · 2016Article
- The clinical outcomes of day 3 4-cell embryos after extended in vitro culture.Journal of assisted reproduction and genetics · 2015Article
- Array comparative genomic hybridization analyses of all blastomeres of a cohort of embryos from young IVF patients revealed significant contribution of mitotic errors to embryo mosaicism at the cleavage stage.Reproductive biology and endocrinology : RB&E · 2014Article
- CDK1 plays an important role in the maintenance of pluripotency and genomic stability in human pluripotent stem cells.Cell death & disease · 2014Article
- Article
- Comparative genomic hybridization selection of blastocysts for repeated implantation failure treatment: a pilot study.BioMed research international · 2014Article
- The Effects of Preimplantation Genetic Testing for Aneuploidy (PGT-A) on Patient-Important Outcomes in Embryo Transfer Cases: A Meta-Analysis.Journal of reproduction & infertilityArticle
- The Effect of Prolonged Culture of Chromosomally Abnormal Human Embryos on The Rate of Diploid Cells.International journal of fertility & sterilityArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionIn order to investigate the dynamics of genomic alterations that occur at different developmental stages in vitro, we examined the chromosome content of human preimplantation embryos by molecular-cytogenetic techniques at the single-cell level, up to 13 days post fertilization.
methodsThe embryos were genetically analyzed several times during their development in culture; each embryo was first analyzed by FISH at 'Day 3' post fertilization, than during its growth in vitro and the third analysis was performed at development arrest, then the entire blastocyst was analyzed by comparative genomic hybridization (CGH/aCGH).
resultsWe found that while on 'Day 3' only 31% of the embryos were detected as normal, on 'Day 5-6', 44% of the embryos were classified as normal and on 'Day 7', 57% were normal. On 'Days 8-13', 52% of the embryos were classified as chromosomally normal. One third of the embryos that were chromosomally abnormal on 'Day 3', were found to be normal at development arrest point. DISCUSSION: These dynamic changes that occur at early developmental stages suggest that testing a single blastomere at 'Day 3' post fertilization for PGD might inaccurately reflect the embryo ploidy and increase the risk of false aneuploidy diagnosis. Alternatively, blastocyst stage diagnosis may be more appropriate.
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