ArticleJournal of the American Society for Mass Spectrometry2013
Efficient methods to generate reproducible mass spectra in matrix-assisted laser desorption ionization of peptides.
Article in Journal of the American Society for Mass Spectrometry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 21 citations in OpenAlex.
- Large-Area Graphene Films as Target Surfaces for Highly Reproducible Matrix-Assisted Laser Desorption Ionization Suitable for Quantitative Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Gain Switching for a Detection System to Accommodate a Newly Developed MALDI-Based Quantification Method.Journal of the American Society for Mass Spectrometry · 2017Article
- Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry.Journal of chromatography. A · 2017Review
- Quantification of Carbohydrates and Related Materials Using Sodium Ion Adducts Produced by Matrix-Assisted Laser Desorption Ionization.Journal of the American Society for Mass Spectrometry · 2016Article
- Investigations of Some Liquid Matrixes for Analyte Quantification by MALDI.Journal of the American Society for Mass Spectrometry · 2015Article
- Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting.Journal of the American Society for Mass Spectrometry · 2014Article
- Why do the abundances of ions generated by MALDI look thermally determined?Journal of the American Society for Mass Spectrometry · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In our previous matrix-assisted laser desorption ionization (MALDI) studies of peptides, we found that their mass spectra were virtually determined by the effective temperature in the early matrix plume, Tearly, when samples were rather homogeneous. This empirical rule allowed acquisition of quantitatively reproducible spectra. A difficulty in utilizing this rule was the complicated spectral treatment needed to get Tearly. In this work, we found another empirical rule that the total number of particles hitting the detector, or TIC, was a good measure of the spectral temperature and, hence, selection of spectra with the same TIC resulted in reproducible spectra. We also succeeded in obtaining reproducible spectra throughout a measurement by controlling TIC near a preset value through feedback adjustment of laser pulse energy. Both TIC selection and TIC control substantially reduced the shot-to-shot spectral variation in a spot, spot-to-spot variation in a sample, and even sample-to-sample variation in MALDI using α-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid as matrix. Based on the utilization of acquired data, TIC control was more efficient than TIC selection by an order of magnitude. Both techniques produced calibration curves with excellent linearity, suggesting their utility in quantification of peptides.
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