ArticleGenome biology and evolution2013
Sequencing angiosperm plastid genomes made easy: a complete set of universal primers and a case study on the phylogeny of saxifragales.
Article in Genome biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
153 citing papers in PubMed, 264 citations in OpenAlex.
- Chloroplast Genome Sequence of Snap Bean (International journal of molecular sciences · 2026Article
- Chloroplast genome sequencing of Poa pratensis and comparative chloroplast genomics analysis with other 29 grass species.Functional & integrative genomics · 2026Article
- Cytotype classification and genetic diversity of Platostoma palustre revealed by rDNA localization and chloroplast genome.BMC genomics · 2025Article
- Insights into chloroplast genome structure and phylogenetic position of the Lacquer tree Toxicodendron trichocarpum (Anacardiaceae: Rhoideae).Journal of plant research · 2025Article
- Article
- Species concept of golden flower camellias in China.BMC plant biology · 2025Article
- De-novo assembly and comparative analysis of the complete mitogenome of traditional Chinese medicine Strobilanthes sarcorrhiza.BMC plant biology · 2025Article
- Comparative and evolutionary analysis of chloroplast genomes from five rare Styrax species.BMC genomics · 2025Article
- Decoding the Chloroplast Genome of Bitterwood (Ecology and evolution · 2025Article
- Comparative chloroplast genomes of Incarvillea species (Bignoniaceae) unveiled genomic diversity and shed light on phylogenetic relationships.BMC plant biology · 2025Article
- Complete Chloroplast Genome Sequences of ThreeCurrent issues in molecular biology · 2025Article
- Intraspecific identification and genetic diversity analysis of Paeonia lactiflora based on chloroplast genes rpoB and psbK-psbI.Molecular biology reports · 2025Article
- The First Complete Chloroplast Genome Sequence ofCurrent issues in molecular biology · 2025Article
- A complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2025Article
- Article
- Comparative analysis of chloroplast genomes in ten holly (Ilex) species: insights into phylogenetics and genome evolution.BMC ecology and evolution · 2024Article
- Comparative analysis of 12 water lily plastid genomes reveals genomic divergence and evolutionary relationships in early flowering plants.Marine life science & technology · 2024Article
- Intraspecific Differentiation ofGenes · 2024Article
- Comparative and phylogenetic analysis of the complete chloroplast genomes of 10 Artemisia selengensis resources based on high-throughput sequencing.BMC genomics · 2024Article
- Phylogenetic relationships, selective pressure and molecular markers development of six species in subfamily Polygonoideae based on complete chloroplast genomes.Scientific reports · 2024Article
93 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastid genomes are an invaluable resource for plant biological studies. However, the number of completely sequenced plant plastid genomes is still small compared with the vast number of species. To provide an alternative generalized approach, we designed a set of 138 pairs of universal primers for amplifying (termed "short-range PCR") and sequencing the entire genomes of the angiosperm plastid genomes. The universality of the primers was tested by using species from the basal to asterid angiosperms. The polymerase chain reaction (PCR) success rate was higher than 96%. We sequenced the complete chloroplast genome of Liquidambar formosana as an example using this method and compared it to the genomes independently determined by long-range PCR (from 6.3 kb to 13.3 kb) and next-generation sequencing methods. The three genomes showed that they were completely identical. To test the phylogenetic efficiency of this method, we amplified and sequenced 18 chloroplast regions of 19 Saxifragales and Saxifragales-related taxa, as a case study, to reconstruct the phylogeny of all families of the order. Phylograms based on a combination of our data, together with those from GenBank, clearly indicate three family groups and three single families within the order. This set of universal primers is expected to accelerate the accumulation of angiosperm plastid genomes and to make faster mass data collection of plastid genomes for molecular systematics.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.