ArticleOpen biology2013
Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.
Article in Open biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 61 citations in OpenAlex.
- Complete genome sequence of the hyperthermophilic archaeonMicrobiology resource announcements · 2025Article
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- High-MOI induces rapid CRISPR spacer acquisition inmicroPublication biology · 2022Article
- Genome editing in archaeal viruses and endogenous viral protein purification.STAR protocols · 2021Article
- A filamentous archaeal virus is enveloped inside the cell and released through pyramidal portals.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- CRISPR-Cas adaptive immune systems in Sulfolobales: genetic studies and molecular mechanisms.Science China. Life sciences · 2021Review
- High-efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme.Microbial biotechnology · 2021Article
- Phenotypic Characterization ofFrontiers in microbiology · 2021Article
- Structural basis for inhibition of an archaeal CRISPR-Cas type I-D large subunit by an anti-CRISPR protein.Nature communications · 2020Article
- DNA targeting by subtype I-D CRISPR-Cas shows type I and type III features.Nucleic acids research · 2020Article
- Structures of filamentous viruses infecting hyperthermophilic archaea explain DNA stabilization in extreme environments.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Duplication of leucyl-tRNA synthetase in an archaeal extremophile may play a role in adaptation to variable environmental conditions.The Journal of biological chemistry · 2020Article
- Types I and V Anti-CRISPR Proteins: From Phage Defense to Eukaryotic Synthetic Gene Circuits.Frontiers in bioengineering and biotechnology · 2020Review
- An extensively glycosylated archaeal pilus survives extreme conditions.Nature microbiology · 2019Article
- The nuts and bolts of the Haloferax CRISPR-Cas system I-B.RNA biology · 2019Article
- Structural conservation in a membrane-enveloped filamentous virus infecting a hyperthermophilic acidophile.Nature communications · 2018Article
- Anti-CRISPR proteins encoded by archaeal lytic viruses inhibit subtype I-D immunity.Nature microbiology · 2018Article
- Spontaneous CRISPR loci generation in vivo by non-canonical spacer integration.Nature microbiology · 2018Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ΔpyrEF and ΔCRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.
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