ArticleAmerican journal of physiology. Renal physiology2013
Real-time electrochemical detection of ATP and H₂O₂ release in freshly isolated kidneys.
Article in American journal of physiology. Renal physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Renin-angiotensin system-mediated nitric oxide signaling in podocytes.American journal of physiology. Renal physiology · 2024Article
- Interaction of Angiotensin II AT1 Receptors with Purinergic P2X Receptors in Regulating Renal Afferent Arterioles in Angiotensin II-Dependent Hypertension.International journal of molecular sciences · 2023Review
- A fluorescence nanoplatform for the determination of hydrogen peroxide and adenosine triphosphate via tuning of the peroxidase-like activity of CuO nanoparticle decorated UiO-66.Mikrochimica acta · 2022Article
- Recent advances in understanding ion transport mechanisms in polycystic kidney disease.Clinical science (London, England : 1979) · 2021Review
- Characterization of purinergic receptor 2 signaling in podocytes from diabetic kidneys.iScience · 2021Article
- NOX4-dependent regulation of ENaC in hypertension and diabetic kidney disease.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Renal sympathetic denervation for resistant hypertension: where do we stand after more than a decade.Jornal brasileiro de nefrologia · 2020Review
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Immune mechanisms of hypertension.Nature reviews. Immunology · 2019Review
- Characterization of purinergic receptor expression in ARPKD cystic epithelia.Purinergic signalling · 2018Article
- A NOX4/TRPC6 Pathway in Podocyte Calcium Regulation and Renal Damage in Diabetic Kidney Disease.Journal of the American Society of Nephrology : JASN · 2018Article
- An investigation into closed-loop treatment of neurological disorders based on sensing mitochondrial dysfunction.Journal of neuroengineering and rehabilitation · 2018Review
- Acute In Vivo Analysis of ATP Release in Rat Kidneys in Response to Changes of Renal Perfusion Pressure.Journal of the American Heart Association · 2017Article
- Renal sodium handling and sodium sensitivity.Kidney research and clinical practice · 2017Review
- Functional and therapeutic importance of purinergic signaling in polycystic kidney disease.American journal of physiology. Renal physiology · 2016Review
- Evidence of the Importance of Nox4 in Production of Hypertension in Dahl Salt-Sensitive Rats.Hypertension (Dallas, Tex. : 1979) · 2016Article
- Podocyte injury in diabetic nephropathy: implications of angiotensin II-dependent activation of TRPC channels.Scientific reports · 2015Article
- Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney.Journal of visualized experiments : JoVE · 2015Article
- TRPC6 channel as an emerging determinant of the podocyte injury susceptibility in kidney diseases.American journal of physiology. Renal physiology · 2015Review
- Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta.Journal of visualized experiments : JoVE · 2015Article
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7 authors at 1 institution in 1 country.
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Abstract
Extracellular nucleotides such as adenosine-5'-triphosphate (ATP) and reactive oxygen species are essential local signaling molecules in the kidney. However, measurements of changes in the interstitial concentrations of these substances in response to various stimuli remain hindered due to limitations of existing experimental techniques. The goal of this study was to develop a novel approach suitable for real-time measurements of ATP and H₂O₂ levels in freshly isolated rat kidney. Rats were anesthetized and the kidneys were flushed to clear blood before isolation for consequent perfusion. The perfused kidneys were placed into a bath solution and dual simultaneous amperometric recordings were made with the enzymatic microelectrode biosensors detecting ATP and H₂O₂. It was found that basal levels of H₂O₂ were increased in Dahl salt-sensitive (SS) rats fed a high-salt diet compared with SS and Sprague-Dawley rats fed a low-salt diet and that medulla contained higher levels of H₂O₂ compared with cortex in both strains. In contrast, ATP levels did not change in SS rats when animals were fed a high-salt diet. Importantly, angiotensin II via AT₁ receptor induced rapid release of both ATP and H₂O₂ and this effect was enhanced in SS rats. These results demonstrate that ATP and H₂O₂ are critical in the development of salt-sensitive hypertension and that the current method represents a unique powerful approach for the real-time monitoring of the changes in endogenous substance levels in whole organs.
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