ArticlePloS one2013
Induced collagen cross-links enhance cartilage integration.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Dose-Dependent, Biomechanical Recovery of Permeability Ex Vivo in GAG-Depleted Cartilage With a Synthetic Polyelectrolyte, Polystyrene Sulfonate.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Hydrogel-Enhanced Autologous Chondrocyte Implantation for Cartilage Regeneration-An Update on Preclinical Studies.Bioengineering (Basel, Switzerland) · 2024Review
- Kinetics and Retention of Polystyrenesulfonate for Proteoglycan Replacement in Cartilage.Biomacromolecules · 2024Article
- A dynamically loadedFrontiers in cell and developmental biology · 2024Article
- Temporal application of lysyl oxidase during hierarchical collagen fiber formation differentially effects tissue mechanics.Acta biomaterialia · 2023Article
- PEG Reinforced Scaffold Promotes Uniform Distribution of Human MSC-Created Cartilage Matrix.Gels (Basel, Switzerland) · 2022Article
- Translating musculoskeletal bioengineering into tissue regeneration therapies.Science translational medicine · 2022Review
- New Insights into Cartilage Tissue Engineering: Improvement of Tissue-Scaffold Integration to Enhance Cartilage Regeneration.BioMed research international · 2022Review
- Article
- Rejuvenation of extensively passaged human chondrocytes to engineer functional articular cartilage.Biofabrication · 2021Article
- Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration.Journal of functional morphology and kinesiology · 2021Review
- Article
- Surgical and tissue engineering strategies for articular cartilage and meniscus repair.Nature reviews. Rheumatology · 2019Review
- Differential Effects of the AntioxidantsCellular and molecular bioengineering · 2019Article
- Temporal development of near-native functional properties and correlations with qMRI in self-assembling fibrocartilage treated with exogenous lysyl oxidase homolog 2.Acta biomaterialia · 2017Article
- The Self-Assembling Process and Applications in Tissue Engineering.Cold Spring Harbor perspectives in medicine · 2017Review
- The Role of the Lysyl Oxidases in Tissue Repair and Remodeling: A Concise Review.Tissue engineering and regenerative medicine · 2017Review
- Matrix Production in Large Engineered Cartilage Constructs Is Enhanced by Nutrient Channels and Excess Media Supply.Tissue engineering. Part C, Methods · 2015Article
- Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods.Journal of the Royal Society, Interface · 2015Article
- Harnessing biomechanics to develop cartilage regeneration strategies.Journal of biomechanical engineering · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Articular cartilage does not integrate due primarily to a scarcity of cross-links and viable cells at the interface. The objective of this study was to test the hypothesis that lysyl-oxidase, a metalloenzyme that forms collagen cross-links, would be effective in improving integration between native-to-native, as well as tissue engineered-to-native cartilage surfaces. To examine these hypotheses, engineered cartilage constructs, synthesized via the self-assembling process, as well as native cartilage, were implanted into native cartilage rings and treated with lysyl-oxidase for varying amounts of time. For both groups, lysyl-oxidase application resulted in greater apparent stiffness across the cartilage interface 2-2.2 times greater than control. The construct-to-native lysyl-oxidase group also exhibited a statistically significant increase in the apparent strength, here defined as the highest observed peak stress during tensile testing. Histology indicated a narrowing gap at the cartilage interface in lysyl-oxidase treated groups, though this alone is not sufficient to indicate annealing. However, when the morphological and mechanical data are taken together, the longer the duration of lysyl-oxidase treatment, the more integrated the interface appeared. Though further data are needed to confirm the mechanism of action, the enhancement of integration may be due to lysyl-oxidase-induced pyridinoline cross-links. This study demonstrates that lysyl-oxidase is a potent agent for enhancing integration between both native-to-native and native-to-engineered cartilages. The fact that interfacial strength increased manifold suggests that cross-linking agents should play a significant role in solving the difficult problem of cartilage integration. Future studies must examine dose, dosing regimen, and cellular responses to lysyl-oxidase to optimize its application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.