ArticlePloS one2013
Engineering of acetate recycling and citrate synthase to improve aerobic succinate production in Corynebacterium glutamicum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Genetically Modified Lactic Acid Bacteria in the EU Food Chain: Applications, Benefits, and Risk Assessment.International journal of molecular sciences · 2026Review
- Succinic Acid Production by Engineered Mannheimia succiniciproducens and Its Use in Chemoenzymatic Poly(Butylene Succinate) Synthesis.Biotechnology and bioengineering · 2025Article
- Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1.Microbial cell factories · 2024Article
- Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1.Biotechnology for biofuels and bioproducts · 2023Article
- Development of a 2-pyrrolidone biosynthetic pathway in Corynebacterium glutamicum by engineering an acetyl-CoA balance route.Amino acids · 2022Article
- Improvement in L-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2022Article
- O-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2022Article
- Improvement of COMicroorganisms · 2021Article
- Enhanced 3-Hydroxypropionic Acid Production From AcetateFrontiers in bioengineering and biotechnology · 2021Article
- Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Metabolic engineering of a fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
- An update of the suicide plasmid-mediated genome editing system in Corynebacterium glutamicum.Microbial biotechnology · 2019Article
- EngineeringBiotechnology for biofuels · 2019Article
- Metabolic engineering ofBiotechnology for biofuels · 2018Article
- Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production.Scientific reports · 2016Article
- Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model.Microbial cell factories · 2016Article
- Metabolic engineering of Escherichia coli for poly(3-hydroxybutyrate) production via threonine bypass.Microbial cell factories · 2015Article
- Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources.Journal of industrial microbiology & biotechnology · 2015Review
- Engineering of Serine-Deamination pathway, Entner-Doudoroff pathway and pyruvate dehydrogenase complex to improve poly(3-hydroxybutyrate) production in Escherichia coli.Microbial cell factories · 2014Article
- Succinate Overproduction: A Case Study of Computational Strain Design Using a Comprehensive Escherichia coli Kinetic Model.Frontiers in bioengineering and biotechnology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium glutamicum lacking the succinate dehydrogenase complex can produce succinate aerobically with acetate representing the major byproduct. Efforts to increase succinate production involved deletion of acetate formation pathways and overexpression of anaplerotic pathways, but acetate formation could not be completely eliminated. To address this issue, we constructed a pathway for recycling wasted carbon in succinate-producing C. glutamicum. The acetyl-CoA synthetase from Bacillus subtilis was heterologously introduced into C. glutamicum for the first time. The engineered strain ZX1 (pEacsA) did not secrete acetate and produced succinate with a yield of 0.50 mol (mol glucose)(-1). Moreover, in order to drive more carbon towards succinate biosynthesis, the native citrate synthase encoded by gltA was overexpressed, leading to strain ZX1 (pEacsAgltA), which showed a 22% increase in succinate yield and a 62% decrease in pyruvate yield compared to strain ZX1 (pEacsA). In fed-batch cultivations, strain ZX1 (pEacsAgltA) produced 241 mM succinate with an average volumetric productivity of 3.55 mM h(-1) and an average yield of 0.63 mol (mol glucose) (-1), making it a promising platform for the aerobic production of succinate at large scale.
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