ArticlePloS one2013
p38 MAPK regulates cavitation and tight junction function in the mouse blastocyst.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Embryonic Mediators of Embryo-Uterus Communication, Implantation and Pregnancy.Molecular reproduction and development · 2026Review
- Primitive means first, not worst: Critical roles for primitive endoderm in embryos and embryo models.Developmental biology · 2025Review
- RBMX2 linkseLife · 2025Article
- Effects of Regulating Hippo and Wnt on the Development and Fate Differentiation of Bovine Embryo.International journal of molecular sciences · 2024Article
- GDF-8 improvesFrontiers in cell and developmental biology · 2024Article
- SINE-Associated LncRNA SAWPA Regulates Porcine Zygotic Genome Activation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Overview of junctional complexes during mammalian early embryonic development.Frontiers in endocrinology · 2023Review
- Association between uterine toxicity induced by chlorpyrifos and downregulation of heparin-binding epidermal growth factor andVeterinary research forum : an international quarterly journal · 2023Article
- Stabilization of F-Actin Cytoskeleton by Paclitaxel Improves the Blastocyst Developmental Competence through P38 MAPK Activity in Porcine Embryos.Biomedicines · 2022Article
- DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification.Open biology · 2021Article
- p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice.Communications biology · 2021Article
- Cell-cell junctions: structure and regulation in physiology and pathology.Tissue barriers · 2021Review
- Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Chlorpyrifos effects on integrin alpha v and beta 3 in implantation window phase.Environmental science and pollution research international · 2020Article
- Spontaneous blastocyst collapse as an embryo marker of low pregnancy outcome: A Time-Lapse study.JBRA assisted reproduction · 2020Article
- Nucleus, Cytoskeleton, and Mitogen-Activated Protein Kinase p38 Dynamics during In Vitro Maturation of Porcine Oocytes.Animals : an open access journal from MDPI · 2019Article
- Human frozen-thawed blastocyst morphokinetics observed using time-lapse cinematography reflects the number of trophectoderm cells.PloS one · 2019Article
- Treatment with AICAR inhibits blastocyst development, trophectoderm differentiation and tight junction formation and function in mice.Molecular human reproduction · 2017Article
- Adiponectin stimulates lipid metabolism via AMPK in rabbit blastocysts.Human reproduction (Oxford, England) · 2017Article
- p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development.Open biology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
unlabelledBlastocyst formation is essential for implantation and maintenance of pregnancy and is dependent on the expression and coordinated function of a series of proteins involved in establishing and maintaining the trans-trophectoderm ion gradient that enables blastocyst expansion. These consist of Na/K-ATPase, adherens junctions, tight junctions (TJ) and aquaporins (AQP). While their role in supporting blastocyst formation is established, the intracellular signaling pathways that coordinate their function is unclear. The p38 MAPK pathway plays a role in regulating these proteins in other cell types and is required for embryo development at the 8-16 cell stage, but its role has not been investigated in the blastocyst. HYPOTHESIS: p38 MAPK regulates blastocyst formation by regulating blastocyst formation gene expression and function.
methodsEmbryos were cultured from the early blastocyst stage for 12 h or 24 h in the presence of a potent and specific p38 MAPK inhibitor, SB 220025. Blastocyst expansion, hatching, gene family expression and localization, TJ function and apoptosis levels were analyzed.
resultsInhibition of the p38 MAPK pathway reduced blastocyst expansion and hatching, increased tight junction permeability, affected TJP1 localization, reduced Aqp3 expression, and induced a significant increase in apoptosis.
conclusionThe p38 MAPK pathway coordinates the overall events that regulate blastocyst formation.
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