ArticlePLoS pathogens2013
Low-volume toolbox for the discovery of immunosuppressive fungal secondary metabolites.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 68 citations in OpenAlex.
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- Regulation and induction of fungal secondary metabolites: a comprehensive review.Archives of microbiology · 2025Review
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A Timeline of Biosynthetic Gene Cluster Discovery inJournal of fungi (Basel, Switzerland) · 2024Article
- A glimpse into the fungal metabolomic abyss: Novel network analysis reveals relationships between exogenous compounds and their outputs.PNAS nexus · 2023Article
- Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived FungusInternational journal of molecular sciences · 2023Article
- Regulation of pseurotin A biosynthesis by GliZ and zinc in Aspergillus fumigatus.Scientific reports · 2023Article
- Lipo-Chitooligosaccharides Induce Specialized Fungal Metabolite Profiles That Modulate Bacterial Growth.mSystems · 2022Article
- How to Completely Squeeze a Fungus-Advanced Genome Mining Tools for Novel Bioactive Substances.Pharmaceutics · 2022Review
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- Natural products in the predatory defence of the filamentous fungal pathogenBeilstein journal of organic chemistry · 2021Review
- Fungal oxylipins direct programmed developmental switches in filamentous fungi.Nature communications · 2020Article
- A comparative genomics study of 23 Aspergillus species from section Flavi.Nature communications · 2020Article
- Host and Pathogen Communication in the Respiratory Tract: Mechanisms and Models of a Complex Signaling Microenvironment.Frontiers in medicine · 2020Review
- Aspergillus fumigatus and Aspergillosis in 2019.Clinical microbiology reviews · 2019Review
- Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Recent advances in the genome mining ofMedChemComm · 2019Review
- A call to arms: Mustering secondary metabolites for success and survival of an opportunistic pathogen.PLoS pathogens · 2019Article
- Fungal secondary metabolism: regulation, function and drug discovery.Nature reviews. Microbiology · 2019Review
- Bifunctional Small Molecules Enhance Neutrophil Activities AgainstFrontiers in immunology · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The secondary metabolome provides pathogenic fungi with a plethoric and versatile panel of molecules that can be deployed during host ingress. While powerful genetic and analytical chemistry methods have been developed to identify fungal secondary metabolites (SMs), discovering the biological activity of SMs remains an elusive yet critical task. Here, we describe a process for identifying the immunosuppressive properties of Aspergillus SMs developed by coupling a cost-effective microfluidic neutrophil chemotaxis assay with an in vivo zebrafish assay. The microfluidic platform allows the identification of metabolites inhibiting neutrophil recruitment with as little as several nano-grams of compound in microliters of fluid. The zebrafish assay demonstrates a simple and accessible approach for performing in vivo studies without requiring any manipulation of the fish. Using this methodology we identify the immunosuppressive properties of a fungal SM, endocrocin. We find that endocrocin is localized in Aspergillus fumigatus spores and its biosynthesis is temperature-dependent. Finally, using the Drosophila toll deficient model, we find that deletion of encA, encoding the polyketide synthase required for endocrocin production, yields a less pathogenic strain of A. fumigatus when spores are harvested from endocrocin permissive but not when harvested from endocrocin restrictive conditions. The tools developed here will open new "function-omic" avenues downstream of the metabolomics, identification, and purification phases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.