Evidence map›Paper›PMID 23592706›Full record

ArticleAdvanced healthcare materials2013

Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes.

John P Frampton, Huilin Shi, Albert Kao, Jack M Parent, Shuichi Takayama

Abstract read
In one paragraph

Article in Advanced healthcare materials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

John P FramptonDepartment of Biomedical Engineering and Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48104, USA.
Huilin Shi
Albert Kao
Jack M Parent
Shuichi Takayama

Funding

Neural Progenitor Grafting for Restorative Stroke TherapyR01NS065450 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PARENT, JACK M · 2010 to 2014
$1.6M
PREDICTIVE GENES, MECHANISMS, AND CLINICAL BIOMARKERS OF SUDEPP20NS076916 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI NOEBELS, JEFFREY · 2011 to 2013
$1.4M
NINDS NIH HHS NS065450NINDS NIH HHS NS076916NINDS NIH HHS P20 NS076916NINDS NIH HHS R01 NS065450
6 · The paper itself

Abstract

Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.

Indexed as

Cell DifferentiationAnimalsCell SeparationCoculture TechniquesDextransFeeder CellsHEK293 CellsHumansInduced Pluripotent Stem CellsMiceMyocytes, CardiacPeptide HydrolasesPolyethylene GlycolsWaterDextransPeptide HydrolasesPolyethylene GlycolsWateraqueous two-phase systemcardiomyocytedextranpolyethylene glycolstem cell

Identifiers

PMID23592706
PMCPMC4103153

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.