ArticleClinical chemistry2013
Microfluidic assay of platelet deposition on collagen by perfusion of whole blood from healthy individuals taking aspirin.
Article in Clinical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- A Synergistic Overview between Microfluidics and Numerical Research for Vascular Flow and Pathological Investigations.Sensors (Basel, Switzerland) · 2024Review
- Point of care whole blood microfluidics for detecting and managing thrombotic and bleeding risks.Lab on a chip · 2021Review
- Vascular protective effect of aspirin and rivaroxaban upon endothelial denudation of the mouse carotid artery.Scientific reports · 2020Article
- Scalable manufacture of a disposable, storage-stable eight-channel microfluidic device for rapid testing of platelet, coagulation, and drug function under whole blood flow.Biomicrofluidics · 2020Article
- Platelet Adhesion and Thrombus Formation in Microchannels: The Effect of Assay-Dependent Variables.International journal of molecular sciences · 2020Article
- Microclot array elastometry for integrated measurement of thrombus formation and clot biomechanics under fluid shear.Nature communications · 2019Article
- Dual antiplatelet and anticoagulant (APAC) heparin proteoglycan mimetic with shear-dependent effects on platelet-collagen binding and thrombin generation.Thrombosis research · 2018Article
- Hemodynamic force triggers rapid NETosis within sterile thrombotic occlusions.Journal of thrombosis and haemostasis : JTH · 2018Article
- Recombinant factor VIIa addition to haemophilic blood perfused over collagen/tissue factor can sufficiently bypass the factor IXa/VIIIa defect to rescue fibrin generation.Haemophilia : the official journal of the World Federation of Hemophilia · 2017Article
- Review
- Transport physics and biorheology in the setting of hemostasis and thrombosis.Journal of thrombosis and haemostasis : JTH · 2016Review
- Systems Analysis of Thrombus Formation.Circulation research · 2016Review
- Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.Experimental hematology · 2015Article
- Article
- Microfluidic technology as an emerging clinical tool to evaluate thrombosis and hemostasis.Thrombosis research · 2015Review
- Review
- Differential impairment of aspirin-dependent platelet cyclooxygenase acetylation by nonsteroidal antiinflammatory drugs.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Detection of platelet sensitivity to inhibitors of COX-1, P2Y₁, and P2Y₁₂ using a whole blood microfluidic flow assay.Thrombosis research · 2014Article
- Microfluidic assay of hemophilic blood clotting: distinct deficits in platelet and fibrin deposition at low factor levels.Journal of thrombosis and haemostasis : JTH · 2014Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMicrofluidic devices can create hemodynamic conditions for platelet assays. We validated an 8-channel device in a study of interdonor response to acetylsalicylic acid (ASA, aspirin) with whole blood from 28 healthy individuals.
methodsPlatelet deposition was assessed before treatment or 24 h after ingestion of 325 mg ASA. Whole blood (plus 100 μmol/L H-d-Phe-Pro-Arg-chloromethylketone to inhibit thrombin) was further treated ex vivo with ASA (0-500 μmol/L) and perfused over fibrillar collagen for 300 s at a venous wall shear rate (200 s(-1)).
resultsEx vivo ASA addition to blood drawn before aspirin ingestion caused a reduction in platelet deposition [half-maximal inhibitory concentration (IC50) approximately 10-20 μmol/L], especially between 150 and 300 s of perfusion, when secondary aggregation mediated by thromboxane was expected. Twenty-seven of 28 individuals displayed smaller deposits (45% mean reduction; range 10%-90%; P < 0.001) from blood obtained 24 h after ASA ingestion (no ASA added ex vivo). In replicate tests, an R value to score secondary aggregation [deposition rate from 150 to 300 s normalized by rate from 60 to 150 s] showed R < 1 in only 2 of 28 individuals without ASA ingestion, with R > 1 in only 3 of 28 individuals after 500 μmol/L ASA addition ex vivo. At 24 h after ASA ingestion, 21 of 28 individuals displayed poor secondary aggregation (R < 1) without ex vivo ASA addition, whereas the 7 individuals with residual secondary aggregation (R > 1) displayed insensitivity to ex vivo ASA addition. Platelet deposition was not correlated with platelet count. Ex vivo ASA addition caused similar inhibition at venous and arterial wall shear rates.
conclusionsMicrofluidic devices quantified platelet deposition after ingestion or ex vivo addition of aspirin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.