ArticleProtein science : a publication of the Protein Society2013
White and green screening with circular polymerase extension cloning for easy and reliable cloning.
Article in Protein science : a publication of the Protein Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Replacing Standard Reporters from Molecular Cloning Plasmids with Chromoproteins for Positive Clone Selection.Molecules (Basel, Switzerland) · 2018Article
- A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking.Protein engineering, design & selection : PEDS · 2017Article
- A designed repeat protein as an affinity capture reagent.Biochemical Society transactions · 2015Review
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2 authors.
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Abstract
Cloning is an essential prerequisite to test protein design and engineering ideas. However, it is often time consuming, unreliable, and therefore frustrating. Here, we present a streamlined cloning strategy that incorporates a powerful white and green screening protocol to identify colonies with inserts. We use circular polymerase extension cloning, which is both ligation and sequence independent. Furthermore, our entire procedure requires only three quick steps and one enzyme making it easy to use, inexpensive, and tractable. We anticipate that this method will be particularly useful for protein engineers who frequently subclone or make focused deletion, insertion, or substitution libraries.
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