ArticlemBio2013
Assembly and annotation of Pneumocystis jirovecii from the human lung microbiome.
Article in mBio, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Precision-cut lung slices as anmBio · 2024Article
- Targeted NGS-Based Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
- RNA Polymerase II Transcription inInternational journal of molecular sciences · 2022Article
- Transmission and Colonization ofJournal of fungi (Basel, Switzerland) · 2021Review
- Evidence of non-tandemly repeated rDNAs and their intragenomic heterogeneity inCommunications biology · 2018Article
- Peltaster fructicola genome reveals evolution from an invasive phytopathogen to an ectophytic parasite.Scientific reports · 2016Article
- Copy Number Variation of Mitochondrial DNA Genes in Pneumocystis jirovecii According to the Fungal Load in BAL Specimens.Frontiers in microbiology · 2016Article
- Diversity of Pneumocystis jirovecii during Infection Revealed by Ultra-Deep Pyrosequencing.Frontiers in microbiology · 2016Article
- Investigating fungal outbreaks in the 21st century.PLoS pathogens · 2015Review
- Pneumocystis jirovecii Rtt109, a novel drug target for Pneumocystis pneumonia in immunosuppressed humans.Antimicrobial agents and chemotherapy · 2014Article
- Multilocus microsatellite genotyping array for investigation of genetic epidemiology of Pneumocystis jirovecii.Journal of clinical microbiology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pneumocystis jirovecii is a fungus that causes Pneumocystis pneumonia in immunosuppressed patients and has been closely associated with AIDS since the beginning of the AIDS epidemic. Because in vitro cultivation of P. jirovecii is not possible, progress has been hindered in our understanding of its life cycle, mode of transmission, metabolic function, and genome. Limited amounts of P. jirovecii can be obtained from infected patients, but the occurrence of bacteria, other fungi, and human cells in clinical samples presents new challenges for whole-genome sequencing and downstream bioinformatic analysis. In a recent article, Cissé et al. used cell immunoprecipitation enrichment together with whole-genome amplification to generate sufficient quantities of DNA for Roche 454 and Illumina sequencing [O. H. Cissé, M. Pagni, and P. M. Hauser, mBio 4(1):e00428-12, 2012, doi:10.1128/mBio.00428-12]. In addition, a bioinformatic pipeline was devised to sort and assemble lung microbiome reads, thereby generating an 8.1-Mb P. jirovecii genome comprised of 356 contigs with an N50 (median length of all contigs) of 41.6 kb. Knowledge of this genome will open new avenues of research, including the identification of nutritional requirements for in vitro cultivation as well as the identification of new and novel drug and vaccine targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.