ArticleNature communications2013
An engineered dimeric protein pore that spans adjacent lipid bilayers.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The Ion Permeability of DNA Nanotube Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Light-controlled synthetic communication networks via paired connexon nanopores.Nature communications · 2025Article
- The minimal chemotactic cell.Science advances · 2025Article
- Encoding extracellular modification of artificial cell membranes using engineered self-translocating proteins.Nature communications · 2024Article
- Article
- Assembly of transmembrane pores from mirror-image peptides.Nature communications · 2022Article
- Review
- Design and Assembly of Transmembrane Helix Barrel.The Journal of membrane biology · 2020Article
- Microfluidic Formation of Honeycomb-Patterned Droplets Bounded by Interface Bilayers via Bimodal Molecular Adsorption.Micromachines · 2020Article
- A large size-selective DNA nanopore with sensing applications.Nature communications · 2019Article
- Synthetic tissues.Emerging topics in life sciences · 2019Article
- Sculpting and fusing biomimetic vesicle networks using optical tweezers.Nature communications · 2018Article
- DNA-assisted oligomerization of pore-forming toxin monomers into precisely-controlled protein channels.Nucleic acids research · 2017Article
- Building membrane nanopores.Nature nanotechnology · 2017Review
- A monodisperse transmembrane α-helical peptide barrel.Nature chemistry · 2017Article
- Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.Scientific reports · 2017Article
- Engineered transmembrane pores.Current opinion in chemical biology · 2016Review
- Semisynthetic protein nanoreactor for single-molecule chemistry.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The bottom-up construction of artificial tissues is an underexplored area of synthetic biology. An important challenge is communication between constituent compartments of the engineered tissue, and between the engineered tissue and additional compartments, including extracellular fluids, further engineered tissue and living cells. Here we present a dimeric transmembrane pore that can span two adjacent lipid bilayers, and thereby allow aqueous compartments to communicate. Two heptameric staphylococcal α-hemolysin pores were covalently linked in an aligned cap-to-cap orientation. The structure of the dimer, (α7)2, was confirmed by biochemical analysis, transmission electron microscopy and single-channel electrical recording. We show that one of two β-barrels of (α7)2 can insert into the lipid bilayer of a small unilamellar vesicle, while the other spans a planar lipid bilayer. The (α7)2 pores spanning two bilayers were also observed by transmission electron microscopy.
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