ArticleJournal of proteome research2013
The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.
Article in Journal of proteome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Activity-dependent COeLife · 2026Article
- Multiomics in silico analysis identifies TM4SF4 as a cell surface target in hepatocellular carcinoma.PloS one · 2025Article
- Mitochondrial Connexins and Mitochondrial Contact Sites with Gap Junction Structure.International journal of molecular sciences · 2023Review
- A High-Throughput Search for SFXN1 Physical Partners Led to the Identification of ATAD3, HSD10 and TIM50.Biology · 2022Article
- Connexin 43 in Mitochondria: What Do We Really Know About Its Function?Frontiers in physiology · 2022Review
- Mechanisms of Connexin Regulating Peptides.International journal of molecular sciences · 2021Review
- Article
- Connexin/Innexin Channels in Cytoplasmic Organelles. Are There Intracellular Gap Junctions? A Hypothesis!International journal of molecular sciences · 2020Article
- Ultrastructure of gap junction and Cx43 expression in gastric cancer tissues of the patients.Archives of medical science : AMS · 2020Article
- Shear stress augments mitochondrial ATP generation that triggers ATP release and CaAmerican journal of physiology. Heart and circulatory physiology · 2018Article
- The role of connexins during early embryonic development: pluripotent stem cells, gene editing, and artificial embryonic tissues as tools to close the knowledge gap.Histochemistry and cell biology · 2018Review
- A Cell Junctional Protein Network Associated with Connexin-26.International journal of molecular sciences · 2018Article
- What's the Function of Connexin 32 in the Peripheral Nervous System?Frontiers in molecular neuroscience · 2018Review
- Connexin hemichannel inhibition reduces acetaminophen-induced liver injury in mice.Toxicology letters · 2017Article
- Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells.American journal of physiology. Cell physiology · 2017Article
- Identification of Novel SCIRR69-Interacting Proteins During ER Stress Using SILAC-Immunoprecipitation Quantitative Proteomics Approach.Neuromolecular medicine · 2017Article
- Connexin-based signaling and drug-induced hepatotoxicity.Journal of clinical and translational research · 2017Article
- Sideroflexin 3 is an α-synuclein-dependent mitochondrial protein that regulates synaptic morphology.Journal of cell science · 2017Article
- Connexins and pannexins in neuronal development and adult neurogenesis.BMC cell biology · 2016Review
- Identification of new FGF1 binding partners-Implications for its intracellular function.IUBMB life · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Connexins are the structural subunits of gap junctions and act as protein platforms for signaling complexes. Little is known about tissue-specific connexin signaling nexuses, given significant challenges associated with affinity-purifying endogenous channel complexes to the level required for interaction analyses. Here, we used multiple subcellular fractionation techniques to isolate connexin32-enriched membrane microdomains from murine liver. We show, for the first time, that connexin32 localizes to both the plasma membrane and inner mitochondrial membrane of hepatocytes. Using a combination of immunoprecipitation-high throughput mass spectrometry, reciprocal co-IP, and subcellular fractionation methodologies, we report a novel interactome validated using null mutant controls. Eighteen connexin32 interacting proteins were identified. The majority represent resident mitochondrial proteins, a minority represent plasma membrane, endoplasmic reticulum, or cytoplasmic partners. In particular, connexin32 interacts with connexin26 and the mitochondrial protein, sideroflexin-1, at the plasma membrane. Connexin32 interaction enhances connexin26 stability. Converging bioinformatic, biochemical, and confocal analyses support a role for connexin32 in transiently tethering mitochondria to connexin32-enriched plasma membrane microdomains through interaction with proteins in the outer mitochondrial membrane, including sideroflexin-1. Complex formation increases the pool of sideroflexin-1 that is present at the plasma membrane. Together, these data identify a novel plasma membrane/mitochondrial signaling nexus in the connexin32 interactome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.