ArticleAnalytical chemistry2013
Microfluidic chemical cytometry of peptide degradation in single drug-treated acute myeloid leukemia cells.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Bone-on-a-Chip Systems for Hematological Cancers.Biosensors · 2025Review
- Microfluidic single-cell measurements of oxidative stress as a function of cell cycle position.Analytical and bioanalytical chemistry · 2023Article
- Design and Application of Sensors for Chemical Cytometry.ACS chemical biology · 2018Review
- Review
- Chemical fixation to arrest phospholipid signaling for chemical cytometry.Journal of chromatography. A · 2017Article
- Novel single-cell technologies in acute myeloid leukemia research.Translational research : the journal of laboratory and clinical medicine · 2017Review
- Rational Design of a Dephosphorylation-Resistant Reporter Enables Single-Cell Measurement of Tyrosine Kinase Activity.ACS chemical biology · 2016Article
- Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Negative dielectrophoretic capture and repulsion of single cells at a bipolar electrode: the impact of faradaic ion enrichment and depletion.Journal of the American Chemical Society · 2015Article
- Single-cell sphingosine kinase activity measurements in primary leukemia.Analytical and bioanalytical chemistry · 2014Article
- Automated single cell microbioreactor for monitoring intracellular dynamics and cell growth in free solution.Lab on a chip · 2014Article
- Measurement of protein kinase B activity in single primary human pancreatic cancer cells.Analytical chemistry · 2014Article
- Response of single leukemic cells to peptidase inhibitor therapy across time and dose using a microfluidic device.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Integrated electroosmotic perfusion of tissue with online microfluidic analysis to track the metabolism of cystamine, pantethine, and coenzyme A.Analytical chemistry · 2013Article
- An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Microfluidic systems show great promise for single-cell analysis; however, as these technologies mature, their utility must be validated by studies of biologically relevant processes. An important biomedical application of these systems is characterization of tumor cell heterogeneity. In this work, we used a robust microfluidic platform to explore the heterogeneity of enzyme activity in single cells treated with a chemotherapeutic drug. Using chemical cytometry, we measured peptide degradation in the U937 acute myeloid leukemia (AML) cell line in the presence and absence of the aminopeptidase inhibitor Tosedostat (CHR-2797). The analysis of 99 untreated cells revealed rapid and consistent degradation of the peptide reporter within 20 min of loading. Results from drug-treated cells showed inhibited, but ongoing degradation of the reporter. Because the device operates at an average sustained throughput of 37 ± 7 cells/h, we were able to sample cells over the course of this time-dependent degradation. In data from 498 individual drug-treated cells, we found a linear dependence of degradation rate on amount of substrate loaded superimposed upon substantial heterogeneity in peptide processing in response to inhibitor treatment. Importantly, these data demonstrated the potential of microfluidic systems to sample biologically relevant analytes and time-dependent processes in large numbers of single cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.