ArticleJournal of the American Chemical Society2013
Genetically encoded multispectral labeling of proteins with polyfluorophores on a DNA backbone.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- A PET reporter ligand for quantitative imaging of gene expression in the brain.Nature biomedical engineering · 2026Article
- Heck-type C-glycosylations in the synthesis of artificial nucleotides and functional nucleic acids.RSC chemical biology · 2026Review
- A Modular Approach for the Synthesis of Diverse Heterobifunctional Cyanine Dyes.The Journal of organic chemistry · 2024Article
- Article
- A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells.STAR protocols · 2022Article
- Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile ZnSTAR protocols · 2021Article
- Synthesis, Optical, Thermal and Structural Characteristics of Novel Thermocleavable Polymers Based on Phthalate Esters.Polymers · 2020Article
- tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging.Royal Society open science · 2019Article
- Protein-Nucleic Acid Conjugation with Sterol Linkers Using Hedgehog Autoprocessing.Bioconjugate chemistry · 2019Article
- Rediscovering Bacteria through Single-Molecule Imaging in Living Cells.Biophysical journal · 2018Review
- Fluorescent nucleobases as tools for studying DNA and RNA.Nature chemistry · 2017Review
- Luminescent Carbon Dot Mimics Assembled on DNA.Journal of the American Chemical Society · 2017Article
- Mechanistic Insight Leads to a Ligand Which Facilitates the Palladium-Catalyzed Formation of 2-(Hetero)Arylaminooxazoles and 4-(Hetero)Arylaminothiazoles.Angewandte Chemie (International ed. in English) · 2017Article
- Color-Change Photoswitching of an Alkynylpyrene Excimer Dye.Angewandte Chemie (International ed. in English) · 2017Article
- Quantum interference and heteroaromaticity of para- and meta-linked bridged biphenyl units in single molecular conductance measurements.Scientific reports · 2017Article
- Applications of Palladium-Catalyzed C-N Cross-Coupling Reactions.Chemical reviews · 2016Review
- Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag.Journal of bacteriology · 2016Article
- HaloTag technology: a versatile platform for biomedical applications.Bioconjugate chemistry · 2015Review
- Localized light-induced protein dimerization in living cells using a photocaged dimerizer.Nature communications · 2014Article
- Fluorescent sensors for measuring metal ions in living systems.Chemical reviews · 2014Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Genetically encoded methods for protein conjugation are of high importance as biological tools. Here we describe the development of a new class of dyes for genetically encoded tagging that add new capabilities for protein reporting and detection via HaloTag methodology. Oligodeoxyfluorosides (ODFs) are short DNA-like oligomers in which the natural nucleic acid bases are replaced by interacting fluorescent chromophores, yielding a broad range of emission colors using a single excitation wavelength. We describe the development of an alkyl halide dehalogenase-compatible chloroalkane linker phosphoramidite derivative that enables the rapid automated synthesis of many possible dyes for protein conjugation. Experiments to test the enzymatic self-conjugation of nine different DNA-like dyes to proteins with HaloTag domains in vitro were performed, and the data confirmed the rapid and efficient covalent labeling of the proteins. Notably, a number of the ODF dyes were found to increase in brightness or change color upon protein conjugation. Tests in mammalian cellular settings revealed that the dyes are functional in multiple cellular contexts, both on the cell surface and within the cytoplasm, allowing protein localization to be imaged in live cells by epifluorescence and laser confocal microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.