ArticleProceedings of the National Academy of Sciences of the United States of America2013
Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 121 citations in OpenAlex.
- Engineered microbial hydrogels with confined architecture and binary microbes for efficient hydrogen production.Nature communications · 2026Article
- Probing substrate water access through the O1 channel of Photosystem II by single site mutations and membrane inlet mass spectrometry.Photosynthesis research · 2025Article
- Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae.International journal of molecular sciences · 2023Article
- Algal cell bionics as a step towards photosynthesis-independent hydrogen production.Nature communications · 2023Article
- Tuning photosynthetic oxygen for hydrogen evolution in synergistically integrated, sulfur deprived consortia of Coccomyxa chodatii and Rhodobium gokarnense at dim and high light.Photosynthesis research · 2023Article
- Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged HPhotosynthesis research · 2022Article
- Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals.Microorganisms · 2022Review
- Characterization of the wave phenomenon of flash-induced chlorophyll fluorescence in Chlamydomonas reinhardtii.Photosynthesis research · 2022Article
- Characterization of the Wave Phenomenon in Flash-Induced Fluorescence Relaxation and Its Application to Study Cyclic Electron Pathways in Microalgae.International journal of molecular sciences · 2022Article
- Interactions Between Carbon Metabolism and Photosynthetic Electron Transport in aFrontiers in plant science · 2022Article
- Water oxidation by photosystem II is the primary source of electrons for sustained HProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Characterization of the transient fluorescence wave phenomenon that occurs during H2 production in Chlamydomonas reinhardtii.Journal of experimental botany · 2019Article
- Omics Application of Bio-Hydrogen Production Through Green AlgaFrontiers in bioengineering and biotechnology · 2019Review
- Z-scheme solar water splittingChemical science · 2018Article
- Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen.Photosynthesis research · 2018Article
- A Stepwise NaHSOFrontiers in plant science · 2018Article
- Evolution of Chlamydomonas reinhardtii ferredoxins and their interactions with [FeFe]-hydrogenases.Photosynthesis research · 2017Review
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Article
- Knock-Down of the IFR1 Protein Perturbs the Homeostasis of Reactive Electrophile Species and Boosts Photosynthetic Hydrogen Production inFrontiers in plant science · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photobiological H2 production is an attractive option for renewable solar fuels. Sulfur-deprived cells of Chlamydomonas reinhardtii have been shown to produce hydrogen with the highest efficiency among photobiological systems. We have investigated the photosynthetic reactions during sulfur deprivation and H2 production in the wild-type and state transition mutant 6 (Stm6) mutant of Chlamydomonas reinhardtii. The incubation period (130 h) was dissected into different phases, and changes in the amount and functional status of photosystem II (PSII) were investigated in vivo by electron paramagnetic resonance spectroscopy and variable fluorescence measurements. In the wild type it was found that the amount of PSII is decreased to 25% of the original level; the electron transport from PSII was completely blocked during the anaerobic phase preceding H2 formation. This block was released during the H2 production phase, indicating that the hydrogenase withdraws electrons from the plastoquinone pool. This partly removes the block in PSII electron transport, thereby permitting electron flow from water oxidation to hydrogenase. In the Stm6 mutant, which has higher respiration and H2 evolution than the wild type, PSII was analogously but much less affected. The addition of the PSII inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea revealed that ∼80% of the H2 production was inhibited in both strains. We conclude that (i) at least in the earlier stages, most of the electrons delivered to the hydrogenase originate from water oxidation by PSII, (ii) a faster onset of anaerobiosis preserves PSII from irreversible photoinhibition, and (iii) mutants with enhanced respiratory activity should be considered for better photobiological H2 production.
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