ArticleProceedings of the National Academy of Sciences of the United States of America2013
Enzymatic transformation of nonfood biomass to starch.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 174 citations in OpenAlex.
- Complete biotransformation of cellulose to starchNational science review · 2026Article
- Production of dicarboxylates from ω-amino acids using a cofactor- and co-substrate-freeEngineering microbiology · 2025Article
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- Loop engineering of enzymes to control their immobilization and ultimately fabricate more efficient heterogeneous biocatalysts.Protein science : a publication of the Protein Society · 2025Article
- Broadening the Substrate Specificity of Cellobiose Phosphorylase fromInternational journal of molecular sciences · 2023Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Contribution of Enzyme Catalysis to the Achievement of the United Nations' Sustainable Development Goals.Molecules (Basel, Switzerland) · 2023Article
- Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes.Advances in biochemical engineering/biotechnology · 2023Article
- A chemoenzymatic cascade with the potential to feed the world and allow humans to live in space.Engineering microbiology · 2022Article
- Fungal Biovalorization of a Brewing Industry Byproduct, Brewer's Spent Grain: A Review.Foods (Basel, Switzerland) · 2021Review
- Efficient Multi-Enzymes Immobilized on Porous Microspheres for Producing Inositol From Starch.Frontiers in bioengineering and biotechnology · 2020Article
- A critical comparison of cellular and cell-free bioproduction systems.Current opinion in biotechnology · 2019Review
- Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products.BMC biotechnology · 2018Article
- Simultaneous biosynthesis of (Metabolic engineering communications · 2018Article
- Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions.Synthetic and systems biotechnology · 2018Article
- AnSynthetic and systems biotechnology · 2018Review
- Carbon-Based Nanomaterials in Biomass-Based Fuel-Fed Fuel Cells.Sensors (Basel, Switzerland) · 2017Review
- AnApplied and environmental microbiology · 2017Article
- Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity.Scientific reports · 2017Article
- Biomanufacturing: history and perspective.Journal of industrial microbiology & biotechnology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The global demand for food could double in another 40 y owing to growth in the population and food consumption per capita. To meet the world's future food and sustainability needs for biofuels and renewable materials, the production of starch-rich cereals and cellulose-rich bioenergy plants must grow substantially while minimizing agriculture's environmental footprint and conserving biodiversity. Here we demonstrate one-pot enzymatic conversion of pretreated biomass to starch through a nonnatural synthetic enzymatic pathway composed of endoglucanase, cellobiohydrolyase, cellobiose phosphorylase, and alpha-glucan phosphorylase originating from bacterial, fungal, and plant sources. A special polypeptide cap in potato alpha-glucan phosphorylase was essential to push a partially hydrolyzed intermediate of cellulose forward to the synthesis of amylose. Up to 30% of the anhydroglucose units in cellulose were converted to starch; the remaining cellulose was hydrolyzed to glucose suitable for ethanol production by yeast in the same bioreactor. Next-generation biorefineries based on simultaneous enzymatic biotransformation and microbial fermentation could address the food, biofuels, and environment trilemma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.