ArticleNucleic acids research2013
Laser capture microdissection-reduced representation bisulfite sequencing (LCM-RRBS) maps changes in DNA methylation associated with gonadectomy-induced adrenocortical neoplasia in the mouse.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- 3D multi-omics tumour atlases: from technology to biology and clinical translation.Nature reviews. Cancer · 2026Review
- Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications.NPJ precision oncology · 2026Review
- Laser-Capture Microdissection Followed by ATAC-Seq.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Mapping cancer biology in space: applications and perspectives on spatial omics for oncology.Molecular cancer · 2024Review
- Chromatin accessibility profiling of targeted cell populations with laser capture microdissection coupled to ATAC-seq.Cell reports methods · 2023Article
- Cell-Free DNA-Methylation-Based Methods and Applications in Oncology.Biomolecules · 2020Review
- RnBeads 2.0: comprehensive analysis of DNA methylation data.Genome biology · 2019Article
- Article
- Epigenomic map of human liver reveals principles of zonated morphogenic and metabolic control.Nature communications · 2018Article
- Cell-type-specific brain methylomes profiled via ultralow-input microfluidics.Nature biomedical engineering · 2018Article
- Age-specific changes in genome-wide methylation enrich for Foxa2 and estrogen receptor alpha binding sites.PloS one · 2018Article
- Assessment of concordance between fresh-frozen and formalin-fixed paraffin embedded tumor DNA methylation using a targeted sequencing approach.Oncotarget · 2017Article
- Multi-tissue DNA methylation age predictor in mouse.Genome biology · 2017Article
- GLI1Molecular and cellular endocrinology · 2017Article
- Performances of Different Fragment Sizes for Reduced Representation Bisulfite Sequencing in Pigs.Biological procedures online · 2017Article
- Genome-wide epigenomic profiling for biomarker discovery.Clinical epigenetics · 2016Review
- Five-aza-2'-deoxycytidine-induced hypomethylation of cholesterol 25-hydroxylase gene is responsible for cell death of myelodysplasia/leukemia cells.Scientific reports · 2015Article
- Toying with fate: Redirecting the differentiation of adrenocortical progenitor cells into gonadal-like tissue.Molecular and cellular endocrinology · 2015Review
- Function and information content of DNA methylation.Nature · 2015Review
- Novel markers of gonadectomy-induced adrenocortical neoplasia in the mouse and ferret.Molecular and cellular endocrinology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
DNA methylation is a mechanism for long-term transcriptional regulation and is required for normal cellular differentiation. Failure to properly establish or maintain DNA methylation patterns leads to cell dysfunction and diseases such as cancer. Identifying DNA methylation signatures in complex tissues can be challenging owing to inaccurate cell enrichment methods and low DNA yields. We have developed a technique called laser capture microdissection-reduced representation bisulfite sequencing (LCM-RRBS) for the multiplexed interrogation of the DNA methylation status of cytosine-guanine dinucleotide islands and promoters. LCM-RRBS accurately and reproducibly profiles genome-wide methylation of DNA extracted from microdissected fresh frozen or formalin-fixed paraffin-embedded tissue samples. To demonstrate the utility of LCM-RRBS, we characterized changes in DNA methylation associated with gonadectomy-induced adrenocortical neoplasia in the mouse. Compared with adjacent normal tissue, the adrenocortical tumors showed reproducible gains and losses of DNA methylation at genes involved in cell differentiation and organ development. LCM-RRBS is a rapid, cost-effective, and sensitive technique for analyzing DNA methylation in heterogeneous tissues and will facilitate the investigation of DNA methylation in cancer and organ development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.