ArticleThe Journal of cell biology2013
Identifying gene locus associations with promyelocytic leukemia nuclear bodies using immuno-TRAP.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- PML modulates epigenetic composition of chromatin to regulate expression of pro-metastatic genes in triple-negative breast cancer.Nucleic acids research · 2023Article
- Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus.eLife · 2023Article
- Review
- Chromatin Hubs: A biological and computational outlook.Computational and structural biotechnology journal · 2022Review
- Review
- PML nuclear bodies and chromatin dynamics: catch me if you can!Nucleic acids research · 2020Review
- Review
- PML2-mediated thread-like nuclear bodies mark late senescence in Hutchinson-Gilford progeria syndrome.Aging cell · 2020Article
- Identification of Genomic Loci Responsible for the Formation of Nuclear Domains Using Lampbrush Chromosomes.Non-coding RNA · 2019Review
- PML Bodies in Mitosis.Cells · 2019Review
- The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.PLoS pathogens · 2019Article
- Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler.The Journal of cell biology · 2018Article
- Higher order genomic organization and regulatory compartmentalization for cell cycle control at the G1/S-phase transition.Journal of cellular physiology · 2018Review
- Visualizing transcription factor dynamics in living cells.The Journal of cell biology · 2018Review
- Chromatin loops and causality loops: the influence of RNA upon spatial nuclear architecture.Chromosoma · 2017Review
- PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX.Genome research · 2017Article
- Myogenic differentiation triggers PML nuclear body loss and DAXX relocalization to chromocentres.Cell death & disease · 2017Article
- PML nuclear bodies contribute to the basal expression of the mTOR inhibitor DDIT4.Scientific reports · 2017Article
- Cajal body function in genome organization and transcriptome diversity.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Important insights into nuclear function would arise if gene loci physically interacting with particular subnuclear domains could be readily identified. Immunofluorescence microscopy combined with fluorescence in situ hybridization (immuno-FISH), the method that would typically be used in such a study, is limited by spatial resolution and requires prior assumptions for selecting genes to probe. Our new technique, immuno-TRAP, overcomes these limitations. Using promyelocytic leukemia nuclear bodies (PML NBs) as a model, we used immuno-TRAP to determine if specific genes localize within molecular dimensions with these bodies. Although we confirmed a TP53 gene-PML NB association, immuno-TRAP allowed us to uncover novel locus-PML NB associations, including the ABCA7 and TFF1 loci and, most surprisingly, the PML locus itself. These associations were cell type specific and reflected the cell's physiological state. Combined with microarrays or deep sequencing, immuno-TRAP provides powerful opportunities for identifying gene locus associations with potentially any nuclear subcompartment.
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Registered trials
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