ArticleAntimicrobial agents and chemotherapy2013
Key role for efflux in the preservative susceptibility and adaptive resistance of Burkholderia cepacia complex bacteria.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 67 citations in OpenAlex.
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- Novel application of metagenomics for the strain-level detection of bacterial contaminants within non-sterile industrial products - a retrospective, real-time analysis.Microbial genomics · 2022Article
- Construction and evaluation of a bioluminescentMicrobiology (Reading, England) · 2021Article
- Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Genomics reveals the novel species placement of industrial contaminant isolates incorrectly identified asMicrobial genomics · 2021Article
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- Isothiazolinone Biocides: Chemistry, Biological, and Toxicity Profiles.Molecules (Basel, Switzerland) · 2020Review
- Understanding the challenges of non-food industrial product contamination.FEMS microbiology letters · 2019Review
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6 authors at 3 institutions in 1 country.
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Abstract
Bacteria from the Burkholderia cepacia complex (Bcc) are encountered as industrial contaminants, and little is known about the species involved or their mechanisms of preservative resistance. Multilocus sequence typing (MLST) revealed that multiple Bcc species may cause contamination, with B. lata (n = 17) and B. cenocepacia (n = 11) dominant within the collection examined. At the strain level, 11 of the 31 industrial sequence types identified had also been recovered from either natural environments or clinical infections. Minimal inhibitory (MIC) and minimum bactericidal (MBC) preservative concentrations varied across 83 selected Bcc strains, with industrial strains demonstrating increased tolerance for dimethylol dimethyl hydantoin (DMDMH). Benzisothiazolinone (BIT), DMDMH, methylisothiazolinone (MIT), a blend of 3:1 methylisothiazolinone-chloromethylisothiazolinone (M-CMIT), methyl paraben (MP), and phenoxyethanol (PH), were all effective anti-Bcc preservatives; benzethonium chloride (BC) and sodium benzoate (SB) were least effective. Since B. lata was the dominant industrial Bcc species, the type strain, 383(T) (LMG 22485(T)), was used to study preservative tolerance. Strain 383 developed stable preservative tolerance for M-CMIT, MIT, BIT, and BC, which resulted in preservative cross-resistance and altered antibiotic susceptibility, motility, and biofilm formation. Transcriptomic analysis of the B. lata 383 M-CMIT-adapted strain demonstrated that efflux played a key role in its M-CMIT tolerance and elevated fluoroquinolone resistance. The role of efflux was corroborated using the inhibitor l-Phe-Arg-β-napthylamide, which reduced the MICs of M-CMIT and ciprofloxacin. In summary, intrinsic preservative tolerance and stable adaptive changes, such as enhanced efflux, play a role in the ability of Bcc bacteria to cause industrial contamination.
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