ArticleThe Journal of physiology2013
Upregulated glial cell line-derived neurotrophic factor through cyclooxygenase-2 activation in the muscle is required for mechanical hyperalgesia after exercise in rats.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The use of BCAA to decrease delayed-onset muscle soreness after a single bout of exercise: a systematic review and meta-analysis.Amino acids · 2021Pooled it
- The Effects of a Phytochemical Supplement Blend on Markers of Exercise-Induced Muscle Damage: A Randomised Controlled Trial.Nutrients · 2026Trial
- A randomized, placebo-controlled, crossover clinical trial to evaluate the effect of a turmeric formulation on muscle soreness and function recovery in moderately active adults.Journal of the International Society of Sports Nutrition · 2025Trial
- The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise.The Journal of physiology · 2017Trial
- Effect of analgesic treatments on voluntary activity, mechanical sensitivity and muscle regeneration after cardiotoxin injection in mice.Scientific reports · 2025Article
- Delayed-Onset Muscle Soreness Begins with a Transient Neural Switch.International journal of molecular sciences · 2025Review
- Article
- Neurochemical mechanism of muscular pain: Insight from the study on delayed onset muscle soreness.The journal of physiological sciences : JPS · 2024Review
- Should We Void Lactate in the Pathophysiology of Delayed Onset Muscle Soreness? Not So Fast! Let's See a Neurocentric View!Metabolites · 2022Article
- Increasing the resting time between drop jumps lessens delayed-onset muscle soreness and limits the extent of prolonged low-frequency force depression in human knee extensor muscles.European journal of applied physiology · 2022Article
- Brief research report: Repurposing pentoxifylline to treat intense acute swimming-Induced delayed-onset muscle soreness in mice: Targeting peripheral and spinal cord nociceptive mechanisms.Frontiers in pharmacology · 2022Article
- Exercise-induced muscle damage: mechanism, assessment and nutritional factors to accelerate recovery.European journal of applied physiology · 2021Review
- Article
- Intense Acute Swimming Induces Delayed-Onset Muscle Soreness Dependent on Spinal Cord Neuroinflammation.Frontiers in pharmacology · 2021Article
- Have We Looked in the Wrong Direction for More Than 100 Years? Delayed Onset Muscle Soreness Is, in Fact, Neural Microdamage Rather Than Muscle Damage.Antioxidants (Basel, Switzerland) · 2020Article
- A dual role for peripheral GDNF signaling in nociception and cardiovascular reflexes in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modulation of exercise-induced muscular damage and hyperalgesia by different 630 nm doses of light-emitting diode therapy (LEDT) in rats.Lasers in medical science · 2019Article
- The relationship between stiffness and pain following unaccustomed eccentric exercise: the effects of gentle stretch and repeated bout.European journal of applied physiology · 2019Article
- Article
- Eccentric Muscle Contractions: Risks and Benefits.Frontiers in physiology · 2019Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unaccustomed strenuous exercise that includes lengthening contraction (LC) often causes delayed onset muscle soreness (DOMS), characterised as muscular mechanical hyperalgesia. Previously we reported that a bradykinin-like substance released from the muscle during exercise plays a pivotal role in triggering the process of muscular mechanical hyperalgesia by upregulating nerve growth factor (NGF) in exercised muscle of rats. We show here that cyclooxygenase (COX)-2 and glial cell line-derived neurotrophic factor (GDNF) are also involved in DOMS. COX-2 inhibitors but not COX-1 inhibitors given orally before LC completely suppressed the development of DOMS, but when given 2 days after LC they failed to reverse the mechanical hyperalgesia. COX-2 mRNA and protein in exercised muscle increased six- to 13-fold in mRNA and 1.7-2-fold in protein 0-12 h after LC. COX-2 inhibitors did not suppress NGF upregulation after LC. Instead, we found GDNF mRNA was upregulated seven- to eight-fold in the exercised muscle 12 h-1 day after LC and blocked by pretreatment of COX-2 inhibitors. In situ hybridisation studies revealed that both COX-2 and GDNF mRNA signals increased at the periphery of skeletal muscle cells 12 h after LC. The accumulation of COX-2 mRNA signals was also observed in small blood vessels. Intramuscular injection of anti-GDNF antibody 2 days after LC partly reversed DOMS. Based on these findings, we conclude that GDNF upregulation through COX-2 activation is essential to mechanical hyperalgesia after exercise.
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