ArticleGigaScience2012
Tissue sampling methods and standards for vertebrate genomics.
Article in GigaScience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 75 citations in OpenAlex.
- From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe.Molecular ecology resources · 2026Review
- Evaluating the Molecular Basis of Nanocalcium-Induced Health Regulation in Zebra Fish (Bioengineering (Basel, Switzerland) · 2025Article
- The first de novo genome assembly and annotation of a green-blooded skink (Prasinohaema aff. flavipes) from a historic museum sample.The Journal of heredity · 2025Article
- Preservation of milk in liquid nitrogen during sample collection does not affect the RNA quality for RNA-seq analysis.BMC genomics · 2025Article
- FOGS: A SNPSTR Marker Database to Combat Wildlife Trafficking and a Cell Culture Bank for Ex-Situ Conservation.Molecular ecology resources · 2025Article
- Optimizing Tongue Fluid Sampling and Testing Protocols for Enhanced PRRSV Isolation from Perinatal Swine Mortalities.Viruses · 2025Article
- A Comparative Study on the Effect of Euthanasia Methods and Sample Storage Conditions on RNA Yield and Quality in Porcine Tissues.Animals : an open access journal from MDPI · 2023Article
- Article
- Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing.GigaScience · 2022Article
- Preserving avian blood and DNA sampled in the wild: A survey of personal experiences.Ecology and evolution · 2022Article
- Cell type-specific analysis by single-cell profiling identifies a stable mammalian tRNA-mRNA interface and increased translation efficiency in neurons.Genome research · 2022Article
- Article
- High-coverage genomes to elucidate the evolution of penguins.GigaScience · 2019Article
- Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000Scientific reports · 2018Article
- A workflow to preserve genome-quality tissue samples from plants in botanical gardens and arboreta.Applications in plant sciences · 2016Article
- Global Genome Biodiversity Network: saving a blueprint of the Tree of Life - a botanical perspective.Annals of botany · 2016Article
- Greater thanPeerJ · 2016Article
- The Genome 10K Project: a way forward.Annual review of animal biosciences · 2015Review
- Building a multi-scaled geospatial temporal ecology database from disparate data sources: fostering open science and data reuse.GigaScience · 2015Review
- A field guide to whole-genome sequencing, assembly and annotation.Evolutionary applications · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 11 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent rise in speed and efficiency of new sequencing technologies have facilitated high-throughput sequencing, assembly and analyses of genomes, advancing ongoing efforts to analyze genetic sequences across major vertebrate groups. Standardized procedures in acquiring high quality DNA and RNA and establishing cell lines from target species will facilitate these initiatives. We provide a legal and methodological guide according to four standards of acquiring and storing tissue for the Genome 10K Project and similar initiatives as follows: four-star (banked tissue/cell cultures, RNA from multiple types of tissue for transcriptomes, and sufficient flash-frozen tissue for 1 mg of DNA, all from a single individual); three-star (RNA as above and frozen tissue for 1 mg of DNA); two-star (frozen tissue for at least 700 μg of DNA); and one-star (ethanol-preserved tissue for 700 μg of DNA or less of mixed quality). At a minimum, all tissues collected for the Genome 10K and other genomic projects should consider each species' natural history and follow institutional and legal requirements. Associated documentation should detail as much information as possible about provenance to ensure representative sampling and subsequent sequencing. Hopefully, the procedures outlined here will not only encourage success in the Genome 10K Project but also inspire the adaptation of standards by other genomic projects, including those involving other biota.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.