ArticleBMC plant biology2013
Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 99 citations in OpenAlex.
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- The Effects of Photoperiodic Transcription Factor OsPRR37 on Grain Filling and Starch Synthesis During Rice Caryopsis Development.Plants (Basel, Switzerland) · 2025Article
- Analysis of nutritional composition in opaque2- and crtRB1-based single- and double-biofortified super sweet corn.Journal of applied genetics · 2025Article
- Multiomics of a rice population identifies genes and genomic regions that bestow low glycemic index and high protein content.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comparative Metabolomics Profiling Reveals Key Metabolites and Associated Pathways Regulating Tuber Dormancy in White Yam (Metabolites · 2023Article
- New semi-dwarfing alleles with increased coleoptile length by gene editing of gibberellin 3-oxidase 1 using CRISPR-Cas9 in barley (Hordeum vulgare L.).Plant biotechnology journal · 2023Article
- Genetic Engineering of Starch Biosynthesis in Maize Seeds for Efficient Enzymatic Digestion of Starch during Bioethanol Production.International journal of molecular sciences · 2023Review
- Effect of ACGT motif in spatiotemporal regulation of AtAVT6D, which improves tolerance to osmotic stress and nitrogen-starvation.Plant molecular biology · 2022Article
- Multiomics approach reveals a role of translational machinery in shaping maize kernel amino acid composition.Plant physiology · 2022Article
- Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes.Scientific reports · 2022Article
- Maize Endosperm Development: Tissues, Cells, Molecular Regulation and Grain Quality Improvement.Frontiers in plant science · 2022Review
- Physiological Responses of Two Contrasting Kiwifruit (Plants (Basel, Switzerland) · 2021Article
- iTRAQ-Based Proteomics Analysis of Response toInternational journal of molecular sciences · 2021Article
- Expression analysis of3 Biotech · 2021Article
- Lysine biofortification in rice by modulating feedback inhibition of aspartate kinase and dihydrodipicolinate synthase.Plant biotechnology journal · 2021Article
- Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.Scientific reports · 2021Article
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Development of Maize Hybrids With Enhanced Vitamin-E, Vitamin-A, Lysine, and Tryptophan Through Molecular Breeding.Frontiers in plant science · 2021Article
- The regulation of zein biosynthesis in maize endosperm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Temporal profiling of essential amino acids in developing maize kernel of normal,Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe opaque2 mutant is valuable for producing maize varieties with enhanced nutritional value. However, the exact mechanisms by which it improves protein quality and creates a soft endosperm texture are unclear. Given the importance of improving nutritional quality in grain crops, a better understanding of the physiological basis for these traits is necessary.
resultsIn this study, we combined transcript profiling and proteomic analysis to better understand which genes and proteins are altered by opaque2 in the W64A inbred line. These analyses showed that the accumulation of some lysine-rich proteins, such as sorbitol dehydrogenase and glyceraldehyde3-phosphate dehydrogenase, was increased in mature kernels and may contribute substantially to the lysine content of opaque2 endosperm. Some defense proteins such as beta-glucosidase aggregating factor were strongly down regulated and may be regulated directly by opaque2. The mutant also had altered expression of a number of starch biosynthesis genes and this was associated with a more highly crystalline starch.
conclusionsThe results of these studies revealed specific target genes that can be investigated to further improve nutritional quality and agronomic performance of high lysine maize lines, particularly those based on the presence of the opaque2 mutation. Alteration of amylopectin branching patterns in opaque2 starch could contribute to generation of the soft, starchy endosperm.
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