ArticleBMC microbiology2013
Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 92 citations in OpenAlex.
- Treatment of biofilms in bacterial vaginosis by an amphoteric tenside pessary-clinical study and microbiota analysis.Microbiome · 2017Trial
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- Investigating Bacterial Vaginosis Pathogenesis Using Peptide Nucleic Acid-FluorescenceOpen forum infectious diseases · 2025Article
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- Prevalence of bacterial vaginosis and its associated risk factors among women of reproductive age attending Jos University Teaching Hospital, Plateau State, Nigeria.GMS hygiene and infection control · 2025Article
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- Therapeutic and diagnostic applications of antisense peptide nucleic acids.Molecular therapy. Nucleic acids · 2024Review
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- State of the Art for Diagnosis of Bacterial Vaginosis.Journal of clinical microbiology · 2023Review
- Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms.NPJ biofilms and microbiomes · 2023Article
- Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis.BMC research notes · 2022Article
- Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases.Diagnostics (Basel, Switzerland) · 2022Review
- Exploiting the Anti-Biofilm Effect of the Engineered Phage Endolysin PM-477 to Disrupt In Vitro Single- and Dual-Species Biofilms of Vaginal Pathogens Associated with Bacterial Vaginosis.Antibiotics (Basel, Switzerland) · 2022Article
- Synergistic effects of carvacrol, α-terpinene, γ-terpinene, ρ-cymene and linalool against Gardnerella species.Scientific reports · 2022Article
- Mechanistic Insights into Immune Suppression and Evasion in Bacterial Vaginosis.Current microbiology · 2022Review
- Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics.Biosensors · 2021Review
- Article
- A New PNA-FISH Probe TargetingFrontiers in cellular and infection microbiology · 2021Article
- Pitfalls Associated with Discriminating Mixed-Species Biofilms by Flow Cytometry.Antibiotics (Basel, Switzerland) · 2020Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluorescence in situ Hybridization assay (PNA FISH) for the detection of Lactobacillus spp. and G. vaginalis in mixed samples.
resultsTherefore, we evaluated and validated two specific PNA probes by using 36 representative Lactobacillus strains, 22 representative G. vaginalis strains and 27 other taxonomically related or pathogenic bacterial strains commonly found in vaginal samples. The probes were also tested at different concentrations of G. vaginalis and Lactobacillus species in vitro, in the presence of a HeLa cell line. Specificity and sensitivity of the PNA probes were found to be 98.0% (95% confidence interval (CI), from 87.8 to 99.9%) and 100% (95% CI, from 88.0 to 100.0%), for Lactobacillus spp.; and 100% (95% CI, from 92.8 to 100%) and 100% (95% CI, from 81.5 to 100.0%) for G. vaginalis. Moreover, the probes were evaluated in mixed samples mimicking women with BV or normal vaginal microflora, demonstrating efficiency and applicability of our PNA FISH.
conclusionsThis quick method accurately detects Lactobacillus spp. and G. vaginalis species in mixed samples, thus enabling efficient evaluation of the two bacterial groups, most frequently encountered in the vagina.
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